isocratic elution

Results Precise separation of the five amino acids was achieved using isocratic elution within 18 minutes .

结果4种氨基酸在18min内洗脱完全，分离效果良好；

The broad peak shown in isocratic elution could be improved in gradient elution .

等度洗脱中相对较宽的色谱峰可以在梯度洗脱中得到改善。

Secondly , type of elution was considered and type of isocratic elution was attempted firstly .

第二是洗脱方式，可先用等度洗脱方式进行尝试。

A rapid and simple HPLC method for the determination of homocysteine in plasma with isocratic elution was established .

本文建立了在等度洗脱下高效液相色谱法（HPLC）测定血清中同型半胱氨酸含量的方法。

Both gradient and isocratic elution are described for rapid selection of starting condition for modifier optimization in reversed-phase HPLC .

本文从梯度和等浓度两方面讨论了反相色谱流动相组分最优化过程中初始条件的迅速选择。

Results : Compared with the isocratic elution method , the gradient elution method could obviously shorten the analytical time from 40 to 16 min.

结果：与等度洗脱法相比，分析运行时间从40min缩短至16min。

The twelve sulfonamides and impurity in sample were completely separated in 22 min with isocratic elution .

该法在等度洗脱下22min之内完成了12种磺胺类药物和样品杂质的分离。

The chromatographic retention behaviors of Prion 113-120 peptide on C18-column were studied under reversed-phase high-performance liquid chromatographic condition with isocratic elution .

以反相高效液相色谱为分析手段，研究了Prion蛋白113－120序列多肽的色谱保留行为。

Results It was showed by PAGE that the differential combination ( chromatography ) of anion and cation exchange had much better purifying ability than anion and cation exchange ( chromatography ) of isocratic elution .

结果：聚丙烯酰胺凝胶电泳考马斯亮蓝染色法结果显示，阴、阳离子交换示差组合色谱法，具有较好的分离纯化效果。

The separation of phosphatidylinositol ( PI ), phosphatidylethanolamine ( PE ), phosphatidylcholine ( PC ) from soybean phospholipids has been researched by using low-pressure column chromatography under isocratic elution .

通过实验探讨了使用低压制备色谱柱在等度洗脱方式下分离大豆磷脂中的磷脂酰肌醇（PI）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）等成分。

The PRPP synthetase activity in BFU Es and CFU Es from bone marrows of mice was estimated by ion pair reversed phase HPLC ( IPr HPLC ), using isocratic elution .

采用离子对反相高效液相色谱法（IPrHPLC）的单液等度洗脱，测定了小鼠骨髓红系爆增性集落形成单位（BFU-Es）与红系集落形成单位（CFU-Es）的PRPP合成酶活性。

Z values ( the slope of the plot of lgk ′ vs.lg1 / B ) of α - amylase in hydrophobic interaction chromatography were measured by isocratic elution under different column temperatures .

测定了不同柱温下α－淀粉酶在疏水色谱法中的Z值（lgk′对作图的斜率）。

Putrescine , spermidine , and spermine can be completely resolved by reversed phase HPLC , isocratic elution with acetonitrile ( 52 % ) through a 5 μ m C 18 column , and detection at 254 nm .

在以流速为1ml／min、流动相为乙腈（52%v／v）、通过5－μMC18反相柱在检测峰为254nm的条件下，腐胺、亚精胺、精胺均能完全溶解。

According to the appearance of tea plant , 45 tea plant resources were screened as candidate . The ester-catechin contents of spring tea shoots , summer tea shoots and autumn tea shoots in 45 cultivars or lines were analyzed by isocratic elution HPLC .

用等度高效液相色谱法，分析检测这45个品种或株系的春梢、夏梢、秋梢酯型儿茶素含量，筛选高酯型儿茶素含量的茶树特异资源。