insertional inactivation

Insertional inactivation of the malate dehydrogenase gene from Bradyrhizobium japonicum

大豆根瘤菌（Bradyrhizobiumjaponicum）苹果酸脱氢酶（mdh）基因的插入失活

Approximately 69 % of the resulting phages were found to be recombinants , based on the insertional inactivation of the lac Z gene of the vector .

根据载体中1acZ基因插入失活的原理，重组子中大约69%为重组噬菌体。

Objective To construct an insertional inactivation mutant of Streptococcus mutans glucan-binding protein D for the further functional study of the protein .

目的利用基因打靶技术构建变异链球菌葡聚糖结合蛋白D基因（gbpD）失活株，用于葡聚糖结合蛋白D基因功能的研究。

By this way we got all of the ten insertional inactivation gene mutants . For each gene , at least six candidate clones were analyzed by Southern blotting .

每个基因随机挑选6个克隆通过southernblotting实验验证，结果表明以上10个基因都不是细菌的必须基因，均可被本研究所采用的突变方法失活。