ficoll

Rat pancreatic islets were microencapsulated with , sodium alginate and poly-lysine , according to Sun 's method after being isolated from pancreatic tissue using modified collagenase digestion and Ficoll density gradient separation .

用改良胶原酶消化技术和聚蔗糖密度梯度离心方法将大鼠胰岛从胰腺分离，按Sun等的方法用海藻酸和聚赖氨酸等予以微囊化。

This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion , Ficoll density gradient separation and hand-picking technique .

用改良胶原酶消化技术，聚蔗糖密度梯度离心及手法挑拾方法从大鼠胰腺成功地分离出完整的胰岛。

The neutrophils were isolated using a Percoll - Ficoll method .

白细胞采用Percoll-Ficoll的方法进行分离。

METHODS : Synaptosomes were prepared from rat cerebral cortex by using differential Ficoll gradients .

方法：大脑皮层突触体通过Ficoll密度梯度离心获取。

Density gradient centrifugation ( Ficoll ), gelatin sedimentation and natural sedimentation were compared .

分别采用Ficoll密度梯度离心、自然沉降和明胶沉降三种方法分离MNC。

Density gradient centrifugation : 1.077 Ficoll was used in the Ficoll separating medium group ;

密度梯度离心法：Ficoll分离液组选用的Ficoll分离液密度为1.077；

Methods Mononuclear cells were isolated from peripheral blood with Ficoll solution and monocytes were obtained by adherent treatment ;

方法用淋巴细胞分离液分离外周血单个核细胞，贴壁法获得单核细胞；

Conclusion Tissue-culture , Ficoll separation and different speed adherence may be the effective method to culture the human trophoblast cell .

结论组织块法培养，Ficoll离心及差速贴壁法纯化是一种较有效的人早孕绒毛滋养细胞培养方法。

Lymphocytes were separated from peripheral venous blood in human beings by the ficoll - isopaque method .

用Ficoll-Iuopaque分离液分离人外周血淋巴细胞，采用放免方法测定了；

Methods : Mesenchymal stem cells were recovered from a ficoll gradient and treated with 5-azacytidine in vitro .

方法：离体实验中，利用ficoll进行密度梯度离心获得间充质干细胞后用5-氮胞苷诱导其分化。

Conclusion Myoblasts could be purified by Ficoll and cultured with DMEM culture liquid contained serum made in our country , in high quantity .

【结果】采用Ficoll纯化以国产血清培养，可获得高产量高纯度的成肌细胞。

The fetal pancrease was resected , digested with Collagenase V and purified with Ficoll solution .

切取胚胎胰腺，胶原酶V消化，纯化。

Methods : Islets were isolated using digestion by ductal injection of collagenase , and purified by centrifugation of Ficoll density gradient .

方法用胶原酶法分离、Ficoll400密度梯度离心法纯化胰岛。

The rat islets were isolated by using collagenase digestive method , purified by Ficoll density gradient centrifugation , and rat hepatocytes isolated simultaneously .

应用胶原酶消化分离大鼠胰岛细胞，以Ficol密度梯度离心纯化，同时分离大鼠肝细胞。

Using Collagenase - Ficoll method , we established a stable ICC cell culture method , which lays a solid foundation for the basic and clinical research in ICC . 2 .

结论1.运用Ⅱ型胶原酶消化-密度梯度离心法，我们建立了稳定的ICC培养方法，获得了相对纯化的单一细胞，为ICC进一步的基础研究奠定了坚实的基础。

Objective To compare density gradient centrifugation using ficoll and the whole bone marrow method as solutes respectively to separate human mesenchymal stem cells ( MSCs ) from bone marrow .

目的比较Ficoll密度梯度离心法分离人骨髓间充质干细胞（MesenchymalStemcells，MSCs）与全骨髓培养法之间的差异，以期建立一种简便、实用的基于临床移植需要的MSCs分离方法。

Objective To compare the isolating efficiency of MNCs from umbilic cord blood ( UCB ) by gelatin-Ficoll and Ficoll alone .

目的比较直接利用Ficoll一步法和明胶-Ficoll两步法分离脐带血MNCs的效果。

Mice whole blood was used to prepare immune serum and Eos in the blood of mice infected with Trichinella was purified by using Ficoll discontinuous density gradient centrifugation .

取小鼠全血，制备免疫血清，并用Ficoll密度梯度离心法分离、提纯感染旋毛虫的小鼠血液中的Eos。

Methods : MSCs were isolated with 3 isolation medium , 1.073g/mL percoll , 1.077g/mL Ficoll and Solution A ( author offer ) .

方法：以Percoll（1.073g/mL）、Ficoll（1.077g/mL）和A液（笔者配制）3种分离介质分离MSCs。

Methods The islets were isolated from rat pancreas by hepatopancreatic duct perfusing with collagenase and purified with Ficoll 400 discontinuous density gradient centrifugation .

方法采用肝胰管内灌注胶原酶消化分离胰岛及Ficoll400密度梯度离心纯化。

Lymphocytes were separated from human peripheral blood by density gradient centrifugation with Ficoll and T cells were activated with polyclonal stimulators PHA and PDB + Ion .

密度梯度法常规分离人外周血淋巴细胞，分别以多克隆刺激剂PHA和PDB+Ion刺激T细胞活化，同时设立未加刺激剂的静止T细胞作对照。

Methods Peripheral blood mononuclear cells ( PBMC ) from 3 cases of normal individuals were isolated by Ficoll and farther selected for CD3 + T cells by MACs negative selection .

方法利用Ficoll分离3例正常人外周血单个核细胞（PBMC），进一步经MACs负性分选CD3+T细胞。

Results : There was no difference in the volume of umbilical cord blood , the density and the number of the nuclear cells between HES and Ficoll method before separating ( P > 0.05 ) .

结果改良HES沉降分离法和Ficoll分离法分离前的脐血量、有核细胞浓度和细胞数差异无统计学意义（P>0.05）；

Mononuclear cells were isolated from mobilized peripheral blood ( MPB ) by density gradient centrifugation over Ficoll . CD34 + cells were purified by using an immunomagnetic bead separation system .

用Ficoll-Hapaque分离法分离动员的外周血（MPB）单个核细胞，免疫磁珠法分离纯化CD34+细胞，并将其在含胎牛血清的液体培养体系中、各组细胞因子诱导下培养15天。

The other 6 rats were used in density gradient centrifugation experiment , which were divided into Ficoll separating medium group and Percoll separating medium group based on different separating medium with 3 rats in each group .

密度梯度离心培养实验取剩余6只，以分离液的不同分为Ficoll分离液组、Percoll分离液组，3只/组。

With FHG medium , the effects of genotypes , culture methods , Ficoll 400 and culture temperatures of dedifferentiation on regeneration frequency of green plant ( RFGP ) in barley were studied .

在基本培养基FHG上研究了基因型、培养方式、Ficoll400及分化培养温度对大麦花粉植株再生频率的影响。

Methods By using a series of techniques , i.e. , stem / progenitor cells culture , expansion in vitro and transplantation in animal model , the hematopoietic activity of fractionated cells in discontinuous Ficoll gradient was evaluated .

方法利用造血干/祖细胞培养、体外扩增、移植动物模型等技术，研究不同比密ficoll分离液分离的脐血造血干/祖细胞的造血活性。

Ficoll density gradient was used to collect and depurate protoplasts . The protoplasts from the 3 rd , and 4 th layer got better results of culture than the 1st , 2nd and 5th layer .

用Ficoll密度梯度离心法收集原生质体进行培养，结果表明第三、四层界面的原生质体有较好的培养效果。

Primordial germ cells ( PGCs ) were isolated from the blood of stage 14-15 chick embryos by using Ficoll density centrifugation . After isolated , primordial germ cells were cultured with or without stroma cells .

以14￣15期鸡胚血液为材料，采用Ficoll密度梯度离心方法，提取鸡胚胎原始生殖细胞（primordialgermcells，PGCs），在无基质细胞和基质细胞上分别进行体外培养。

Aim To proliferate and identify dendritic cells from the peripheral blood of patients with chronic hepatitis B.Methods Peripheral blood mononuclear cells ( PBMC ) were isolated from normal donors and patients by centrifugation in Ficoll Hypaque .

目的尝试从慢性乙型肝炎患者外周血扩增培养出大量树突细胞，为进一步临床应用树突细胞治疗慢性乙型肝炎提供物质基础。