f12

Group B : fibroblasts + Schwann cells + DMEM / F12 ;

B组：成纤维细胞+雪旺细胞+DMEM/F12；

Control group III ( NSC + DMEM / F12 ) .

试验对照组3（NSC+DMEM/F12）。

Control group I ( SC medium + NSC + DMEM / F12 );

试验对照组1（SC培养基+NSC+DMEM/F12）；

THC imports f12 years , specialized dedicated , is worth trusting !

天浩诚进口十二年，专业专注，值得信赖！

Control group : ( DMEM / F12 + 10 % FBS ), test group : brain tissue extracts .

分为对照组（DMEM/F12+10%FBS）和脑组织匀浆上清组。

The culture results of the porcine ear skin fibroblasts was good by using DMEM / F12 containing 20 % fetal bovine serum .

在培养条件方面选取DMEM／F12培养液+20%胎牛血清培养猪耳皮肤成纤维细胞，效果较好。

Conclusion : Human umbilical vessel ring culture model of three dimensions in DMEM / F12 ( 1:1 ) serum-free medium has been established .

结论：用无血清培养基和Ⅰ型胶原可以立体培养脐血管环。

After digestion , cells were cultured in DMEM / F12 supplemented with 20 % fetal calf serum .

将消化后得到的细胞用含20%胎牛血清的DMEM/F12培养基进行培养。

Developer 's tools are built in ( just hit F12 ) if you want to dig into the DOM or measure performance .

浏览器还内置了开发者工具（只要按F12即可），如果你有兴趣深挖DOM（文档对象模型）或测试性能。

Methods PC12 cell was irradiated with DMEM / F12 agent of enriched uranium , and the internal exposure doses were calculated .

方法在PC12细胞中加入浓缩铀DMEM/F12工作液，计算PC12细胞的内照射吸收剂量。

Otherwise object number two could never be accelerated I call that force f12 the force that one exerts on two1 I know that number two has an acceleration of one .

否则物体二是不可能有加速度的，记这个推力为F12，表一对二的力，二号物体加速度为。

The collected fat mesenchymal stem cells and cell nucleus pulposus with DMEM / F12 ( 1 : 1 ) medium monolayer culture respectively . 2 .

所收集的脂肪间充质干细胞和髓核细胞用DMEM/F12（1：1）培养液分别单层培养。

The results showed that DMEM / F12 was a good cell culture medium for the epidermal stem cells and the epidermal stem cells can survive to the11 subculture in vitro .

结果表明，DMEM／F12是一种适合表皮干细胞体外增殖培养的培养基，在此培养体系中细胞可传代至11代。

Methods Nucleus pulposus tissue taken from a one-month-old rabbit was treated by Trypsin and collagenase , and then the cells were cultured in DMEM / F12 medium .

方法取一月龄新西兰兔的髓核，胰酶和胶原酶消化，DMEM/F12培养基中培养，倒置显微镜观察细胞形态。

Samples in the normal control group and the model control group were cultured in serum DMEM / F12 ( containing 0.2 fetal calf serum ) without growth factor for 7 days to induce the differentiation .

正常对照组和模型对照组在加入去除生长因子的血清DMEM/F12（含体积分数为0.2的胎牛血清）中培养7d进行诱导分化。

Basal medium was composed of penicillinum , phytomycin , amphotericin B and DMEM / F12 containing B27 annex solution of 0.02 volume fraction .

基础培养基组成：含青霉素、链霉素、两性霉素B及体积分数为0.02B27添加液的DMEM/F12。

The proliferation and differentiation features of NSCs were observed under phase contrast microscope after the GM1 with different concentrations were added into NSCs ' and serum-free DMEM / F12 culture media .

2在NSCs培养基和DMEM/F12培养基中加入不同浓度的GM1，观察GM1对NSCs增殖和分化的影响。

The cells in the control group were added with DMEM / F12 nutrient fluid of EGF , bFGF and B27 to culture in 5 % CO2 incubator for one week at 37 ℃ .

对照组细胞中直接加含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液，37℃，体积分数为0.05的CO2培养箱培养1周。

Umbilical artery rings and umbilical vein rings embedded in three-dimensional collagen gels in DMEM / F12 ( 1:1 ) serum-free medium grew well . The new outgrows were positive by CD34 immunohistochemistry .

脐动脉环和脐静脉环在胶原凝胶和DMEM/F12（1：1）无血清培养基中生长良好，CD34免疫组织化学鉴定其为新生的血管。

Methods The endometrial stromal cells were cultured in DMEM / F12 medium containing different concentrations of IL-6 or IL-8 for 24 hours , then the media were collected to measure the levels of MMP-9 by ELISA .

方法分别用含不同浓度的IL-6和IL-8以及无干扰因素的培养液培养各组人子宫内膜基质细胞24h后，ELISA方法测定培养液中MMP-9的含量。

When corpus striatum extract + DMEM / F12 ( 1:1 ) used as induction media ( corpus striatum group ), 14.49 % diencephalons NSCs was differentiated into dopaminergic neuron , significantly lower than inducer group .

用纹状体提取液与DMEM/F12按1∶1的比例组成的诱导液将中脑来源的14.49%的神经干细胞诱导为多巴胺能神经元，低于诱导剂组诱导分化的多巴胺能神经元。

NSC isolated from two months old rat ′ s brain region like hippocampus and striatum was cultivated in a DMEM / F12 medium containing EGF and bFGF , and was identified with morphological character and nestin immunocytochemistry test .

将从2个月龄大鼠脑海马、纹状体等区域分离的细胞培养于含EGF和bFGF的DMEM/F12培养液中，在光镜下观察细胞的形态特征，并行神经巢蛋白（nestin）细胞化学染色。

The pipe is made of steel X20CrMoV121 ( F12 ) . According to stress analysis and metallographic examination results , a concluding evaluation has been obtained and guides for further safe service set up , together with a residual life expectancy estimation .

该管道的材料是X20CrMoV121（F12），通过应力分析、金相组织检查等方法对该管道的性能进行评估，提出了合理的运行安全性指导意见和剩余寿命估算值。

Conclusion The slices from 2 or 4 week-old rat hippocampi and DMEM / F12 medium may be the preferred choice for tau associated researches . An ideal Alzheimer 's disease model may be established based on the results of these researches .

结论选取2或4周龄大鼠，应用培养基DMEM/F12更适合于脑片水平tau蛋白的相关研究，在此基础上可望建立阿尔茨海默病理想研究模型。

Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells . A group : DMEM / F12 contained 10 % calf blood serum . B group : conditioned medium of macrophages activated by self-neural homogenate .

方法取3-4dSD乳鼠坐骨神经，纯化培养许旺细胞，A组加入含10%胎牛血清的DMEM/F12培养基，B组加入自体神经匀浆激活的巨噬细胞条件培养基；

Methods NSCs harvested from the cerebral cortex of neonatal one-week mice were triturated and cultivated in serum-free DMEM / F12 + bFGF + EGF + B27 , then identified by the immunohistochemistry SABC method .

方法取新生1周内乳鼠的大脑皮质，机械分离出神经干细胞，无血清培养液（DMEM/F12+bFGF+EGF+B27）培养，免疫组化SABC法对神经干细胞进行鉴定；

The length of process in the fetal calf serum + DMEM / F12 group was longer significantly than that in the newly calf born serum + fetal calf serum + DMEM / F12 group in 4 days ( P < 0.01 );

在4d内胎牛血清+DMEM/F12组细胞突起长度明显优于新生牛血清+胎牛血清+DMEM/F12组（P<0.01）；

At the third-generation ( F3 ) cells continued to be cultured ( 10 % FBS , DMEM / F12 ), and the cell population is divided into intervention group and control group , the experimental group were added to different concentrations of bFGF .

取第3代（F3）细胞继续进行培养（10%FBS，DMEM/F12），并将该细胞群分为干预组和空白对照组，实验组分别加入不同浓度梯度的bFGF。

Rat fetal neural stem cells ( rFNSCs ) was separated from embryo about 14.5 ~ 16.5 days , and cultured in DMEM / F12 media with additives and epidermal growth factor ( EGF ) and basic fibroblast growth factor ( bFGF ) .

从14.5～16.5d的大鼠胚胎分离神经干细胞，培养于添加相应成分以及表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的DMEM/F12培养液中。

Methods The tissues of nucleus of inferior colliculus in newborn mice were isolated , collected and cultured with condition serum-free medium ( DMEM / F12 , B27 , EGF 20 ng / ml and bFGF 20 ng / ml ) in vitro .

方法分离、收集新生昆明小鼠下丘核区脑组织，体外经EGF和bFGF刺激下培养；