electrotransformation

Methods of increasing efficiency of electrotransformation of B.flavum C-11

提高黄色短杆菌C-11电转化效率的方法

A post pulse recovery time of 2 ~ 3h and the use of sub inhibitory concentrations of antibiotics in the selective plates were very critical for electrotransformation .

同时，发现电击后的细胞必需在高渗培养基中才能存活，电击后2～3h的复苏表达期和用亚抑制抗生素浓度选择转化子，这些对电转化成功以及提高转化效率都是十分关键的。

Effect of High Osmolarity on Electrotransformation Efficiency of Bacteria

高渗透压对细菌电转化率的影响

By the application of electrotransformation technology , an effective transgenic breeding system of Agaricus bisporus was built .

应用电击转化技术，建立起一套有效的双孢蘑菇转基因育种体系。试验结果表明，运用电击法已成功地把外源基因转入双保蘑菇原生质体中并得到表达。

Treatment of the recipient Lactobacilli with ampicillin improved electrotransformation efficiencies up to 200 fold .

受体细胞经20μg／ml氨苄青霉素处理1h，可以提高转化效率200倍。

A human phage antibody library consisting of 1.3 × 106 clones was constructed by electrotransformation and 60 percent of the clones contained Fab genes .

电转化构建的噬菌体抗体库的库容为1.3×106，Fab基因的重组率为60%。