dnastar
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Sequence data were compiled and analyzed by using the sequence analysis software package ( DNASTAR ) .
用DNASTAR分析软件包进行序列的数据编辑、翻译、进化树的绘制和遗传演化分析。
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Methods The total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into T vector , sequenced and analyzed by DNASTAR software .
方法设计出针对各片段的特异性引物,用PCR方法从Z37病毒感染的细胞提取细胞总RNA,逆转录扩增、产物克隆T载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。