dithiothreitol

No dimmers / oligomers were detected when the refolding and unfolding solution contained the reducing agent dithiothreitol ( DTT ) .

在氧化重折叠反应液中加入还原剂二硫苏糖醇（DTT）后，不但没有分子间二硫键形成，也没有异肽键形成。

Dithiothreitol ( DTT ) could significantly improve the recovery and velocity of frozen-thawed spermatozoa when it was added in the protective medium .

在精子冷冻保存液中加入二硫苏糖醇（DTT），观察复温后精子复苏率、运动速度、测定精子内ATP和精浆内GOT含量。

The disulfide bond reducing agent dithiothreitol ( DTT ) significantly blocked As 2O 3 induced ΔΨ m collapse and apoptosis .

巯基还原剂&二巯基苏糖醇（DTT）显著抑制As2O3诱导的ΔΨm下降。

The inclusion bodies of 3A were dissolved in 8 mol / mL urea buffer , and dithiothreitol ( DTT ) was added as reducing agent .

在8mol/mL尿素溶液中，以二硫苏糖醇（DTT）为还原剂，对基因工程血管抑素3A包涵体进行溶解实验。

The kinetics of iron released from the ferric core of horse spleen ferritin ( HSF ) by dithiothreitol ( DTT ) was determined by electronic spectroscopy method .

用光谱法研究了二硫苏糖醇（DTT）还原马脾铁蛋白（HSF）释放铁的动力学。

Method Reduction with dithiothreitol and Pre-column derivation with o-phthalaldehyde followed by high-performance liquid chromatographic ( HPLC ) method with a short or long reversed-phase C18 column and fluorescence detection are described .

方法用二硫苏糖醇还原，邻苯二醛柱前衍生，用长（150mm）或短（30mm）反相C18柱色谱分离，再用荧光检测器测定血浆GSH。

Objective to evaluate the application of dithiothreitol ( DTT ) instead of 2-Mercaptoethanol ( 2-me ) in the detection of anti-type A ( or B ) IgG antibody .

目的：评价用二硫苏糖醇代替2巯基乙醇灭活IgM抗体在IgG抗A（B）测定中的应用。

At first , RNase A was denatured by urea and dithiothreitol ( DTT ) . Effect of strong denaturant urea and DTT on enzyme activity and free thiol number was investigated .

首先利用脲和DTT作为变性剂，研究了脲和DTT浓度对RNaseA的酶活和自由巯基的影响规律。

A significant decline in the formaldehyde accumulation in the presence of either reduced glutathione or dithiothreitol suggested generation of a free radical species as an initial oxidation intermediate during the demethylation of aminocarb by SLO .

还原型谷胱甘肽（GSH）或二硫苏糖醇参与反应时，甲醛的累积量明显降低，表明SLO对灭害威脱甲基作用时，产生自由基。

The preparation of one-step kit for the 99mTc labeled human IgG with dithiothreitol reduction and a comparison of the one-step method with the ligand exchange method were reported , The stability was good , and labeling efficiency was over 95 % .

采用二硫苏糖醇还原IgG，研制成99mTc－IgG一步法药盒，并与配体交换二步法标记进行对比。

Dithiothreitol ( DTT ) modification , NR / R one-dimensional SDS-PAGE and NR / R two-dimensional SDS-PAGE showed there is no disulfide bond in sucrase . Activity differences of the enzyme were determined when metal ions effected on the sucrase .

二巯基苏糖醇（DTT）修饰，NR／R单向SDS-PAGE、NR／R双向对角线SDS-PAGE的结果均表明蔗糖酶分子内没有二硫键存在。

The oxidation of quantum dots cores was blocked by addition of an appropriate dose of strong reducing agents ( dithiothreitol , ethyl gallate ) and by efficient oxygen removal using sodium sulfite , and as a result , blue shift of quantum dots was suppressed .

添加一定浓度的强还原剂二硫代苏糖醇和没食子酸乙酯，以及对溶液进行除氧后阻止了量子点核的氧化，从而抑制了量子点的蓝移。

The thio-dependent activation of Fru-2 , 6-P_2 to tomato fruit PFP has been investigated . The enzyme partially purified in the absence of dithiothreitol almost lost its sensitivity to Fru-2 , 6-P_2 over the range from pH 6.0 to 9.0 ( Fig. 2 ) .

在无二硫苏糖醇（DTT）存在下得到部份纯化的氧化型PFP酶，在广泛的pH范围内（pH6.0～9.0）失去其大部分对果糖2,6-二磷酸的敏感性。