deoxyuridine
- n.脱氧尿苷;脱氧尿嘧啶核苷;嘧啶核苷;尿嘧啶脱氧核苷
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Methods The murine local lymph node assay based on 5-bromo-2 ′ - deoxyuridine ( BrdU ) incorporation and Buehler test were used to test 2,4-dinitrochlorobenzene ( DNCB ) and geoline allergenicity .
方法采用5-溴-2′-脱氧尿苷结合的小鼠局部淋巴结细胞免疫化学方法和豚鼠局部封闭涂皮试验(Buehlertest)分别检测2,4-二硝基氯代苯和凡士林的致敏性。
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The preparation of sample : The rats in the two groups were treated with 5-bromium deoxyuridine 50 mg / kg , the marker of proliferation cells , through abdominal cavity for administration 3 times and once every 5 hours one day before the corresponding time point .
标本制备:两组大鼠于相应时间点前1d以增殖性细胞的标记物5-溴脱氧尿苷50mg/kg腹腔注射给药3次,间隔4h1次。
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Study on lethal effect of [ 5 - ~ ( 125 ) I ] Iodo-2 ′ - deoxyuridine on rat C _6 glioma cells
5-~(125)I-2′脱氧尿嘧啶核苷杀伤大鼠C6细胞的实验研究
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Conclusion The murine local lymph node assay based on 5-bromo-2 ′ - deoxyuridine incorporation can be used as an alternative methods to test chemicals allergenicity .
结论小鼠局部淋巴结免疫化学方法可替代豚鼠致敏性实验方法用于评价化学物的致敏性。
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We observed apoptosis with terminal transferase deoxyuridine nick-end assays and tested NO of the prostate tissue with nitrite reduction test then calculated the activity of NOS.
通过原位末端检测技术测定前列腺组织细胞凋亡情况;应用亚硝酸盐还原酶法检测前列腺组织中NO变化并计算NOS活力的变化;
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Methods The third passage 5-bromo-2 ' deoxyuridine ( BrdU ) labeled fibroblasts which were isolated from fetal rabbits were seeded on HA · ECM .
方法从胎兔分离的成纤维细胞用5溴脱氧尿嘧啶核苷(BrdU)标记后种植于人羊膜细胞外基质上。
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The rates of purity and proliferation of cultured Schwann cells were assessed by S 100 and 5 bromo 2 deoxyuridine ( BrdU ) immunostaining .
用S100和5溴化鸟嘧啶(BrdU)免疫组化法分别鉴定雪旺细胞的纯度和增殖率。
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Experimental Study of 5 - [ ~( 125 ) I ] iodo-2 - deoxyuridine for Treating Lung Cancer ; Immunohistochemical Detection of Labeling Skin of Inner Mongolia Cashmere Goats with BrdU
~(125)I-脱氧尿嘧啶核苷治疗肺癌的实验研究5-溴-2-脱氧尿嘧啶核苷标记内蒙古绒山羊皮肤组织的免疫组织化学检测
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Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas ( TDT ) mediated deoxyuridine triphosphate ( dUTP ) biotin nick ending labeling ( TUNEL );
用DNA缺口原位末端标记(TUNEL)技术检测细胞凋亡;
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Objective To study the pharmacokinetics and tissue distribution after intravesical instillation of 5 - [ 125I ] iodo-2 ' - deoxyuridine ( 125I-UdR ) in rat bladder .
目的探讨125I-脱氧尿嘧啶核苷(125I-UdR)在大鼠膀胱内灌注后药代动力学及组织分布情况。
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Methods : Apoptotic spermatogenic cells were examined by Wright Giemsa staining and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate ( dUTP ) biotin nick end labeling ( TUNEL ) technique .
方法:采用瑞-姬染色和末端脱氧核苷酸转移酶(TdT)介导的脱氧核苷酸原位末端标记法(TUNEL)检测凋亡生精细胞。
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Pathological results of heart samples in the groups : Much of the 5-bromo - 2 ' - deoxyuridine ( BrdU ) labelled mononuclear cells were identified in the scar tissue , and the positive rate was about 50 % .
各组犬心脏标本病理研究结果:骨髓单个核细胞经5-溴脱氧尿嘧啶核苷标记的阳性率约为50%;
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In situ hybridization was performed by the RNA probe of PDGF-A and rat pancreatic elastase which was labeled with digoxin , and the double immunostaining for 5-bromo-2 ' - deoxyuridine ( BrdU ) was performed .
以血小板源性生长因子A和大鼠胰腺丝氨酸弹性蛋白酶的地高辛标记RNA探针进行原位杂交,并用抗5溴脱氧尿嘧啶抗体进行双重染色。
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Compared with primary culture , the purifying culture by the differential velocity adherent technique combined with 5 ' bromo 2 ' deoxyuridine treatment can more obviously increase the proportion of sinoatrial node fusiform cells , so it is a reliable culture technique of sinoatrial node cells .
因此用差速贴壁结合5-溴脱氧尿核苷处理的纯化培养较常规培养可更明显提高窦房结梭形细胞的比例,是一种可靠的窦房结细胞培养技术。
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Objective To investigate the effect of 5 - iodo-2 ′ - deoxyuridine ( 125IUdR ) on the expressions of argyrophilic nucleolar organizer regions ( AgNOR ) proteins and proliferating cell nuclear antigen ( PCNA ) of C6 glioma cells in Rats .
目的探讨5-[125I]-2'-脱氧尿苷(125IUdR)对大鼠C6胶质瘤细胞中嗜银核仁组织原区蛋白(AgNOR蛋白)和增殖细胞核抗原(PCNA)表达的影响。
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The contents of NO and NOS were measured histochemically . Apoptosis of myocardium was detected for apoptosis index ( AI ) as the index of ischemic reperfusion injury with in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling ( TUNEL ) .
应用组织化学方法测定心肌组织中NO、NOS的含量,应用核酸原位末端标记法(TUNEL)测定心肌细胞凋亡指数,作为评价心肌缺血再灌注损伤的指标。
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The electrophysiological characteristic of the sinoatrial node fusiform cells purified by the differential velocity adherent technique combined with 5 ' bromo 2 ' deoxyuridine treatment was in accordance with that of sinoatrial node pacemaker cells , that was , action potential had diastolic spontaneous depolarization ;
差速贴壁结合5-溴脱氧尿核苷处理的纯化培养的窦房结细胞梭形细胞电生理特征符合窦房结起搏细胞的特点,即动作电位有舒张期自动去极化。
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Histopathological rejection grading in the grafted tissue was performed with hematoxylin eosin ( HE ) staining . Apoptosis cells were detected by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling ( TUNEL ), and the apoptosis index ( AI ) was calculated .
苏木精-伊红(HE)染色进行移植组织排斥反应的病理分级,原位末端标记(TUNEL)法检测移植组织细胞的凋亡情况,计算凋亡指数。
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Objective : To investigate the distribution of radioactivity after intravesical instillation of 5 - [ 125I ] iodo-2 ' - deoxyuridine ( 125I-UdR ) in bladder of normal rats and investigate the distribution and safety of 125I-UdR in rat bearing bladder cancer through pathology study .
目的:通过病理学研究了解125I-脱氧尿嘧啶核苷(125I-UdR)在正常大鼠膀胱内灌注后组织分布情况以及125I-UdR在膀胱癌大鼠模型中的分布和用药安全性。
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Methods The bone marrow was collected from Wistar rats by the flushing of the femurs , MSCs were isolated and purified by the density gradient centrifugation . Then , the MSCs were amplified and labelled with 5-bromo-2 ′ - deoxyuridine ( BrdU ) .
方法无菌条件下取Wistar大鼠股骨骨髓细胞,密度梯度离心分离、纯化MSCs,体外培养扩增后,用BrdU标记细胞。
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Methods Injection of the thymidine analog 5 bromo 2 deoxyuridine ( BrdU ) to determine the extent of cell proliferation combined with single or double immunohistochemistry staining with antibodies BrdU , TuJ1 ( class ⅲ β tubulin , neuron specific early differentiation marker ) and GFAP .
方法5溴2脱氧尿苷(BrdU)标记新生细胞结合免疫组织化学方法对脑切片进行BrdU、TuJ1及GFAP单标或双标染色。
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Ten nucleosides and bases were effectively separated . Five nucleosides ( deoxycytidine , deoxyuridine , 7-methylguanosine , inosine , pseudouridine ) and one free base ( thymine ) were shown to increase significantly soon after irradiation , and then gradually to decrease in the following days .
照后尿中5种核苷(脱氧胞苷、脱氧尿苷、7-甲基鸟苷、肌苷、假尿苷)和一种游离的碱基(胸腺嘧啶)大幅度增加,随后在一周内逐渐减少。
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The cardiac grafts were harvested at 3rd and 5th day after transplantation . The cardiac allograft sections were subjected to the HE staining and in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling ( TUN EL ) for histopathological rejection grade and apoptotic index ( AI ) .
采用HE染色和原位末端标记(TUNEL)技术检测移植心切片,进行排斥反应的病理分级并计算移植心肌细胞的凋亡指数(AI)。