closed circular DNA

A Simple Method for Isolation of Covalently Closed Circular DNA Molecules

一种简易的抽提共价闭合环状DNA的方法

Evaluation of the Value of Quantitative Assay of Hepatitis B Virus Covalently Closed Circular DNA in Diagnosis and Therapy of Chronic Hepatitis B

慢性乙型肝炎共价闭合环状DNA定量测定在诊断和治疗中的价值

Covalently closed circular DNA ( cccDNA ), the primary template for viral replication , is a key factor for persistent infection .

HBV共价闭合环状DNA（covalentlyclosedcircularDNA，cccDNA）作为HBV基因组的原始复制模板，是HBV持续感染的关键因素。

Once HBV has entered the host cell , its genome is converted into a covalently closed circular DNA ( CCC-DNA ) .

当HBV进入宿主细胞后，基因组转变为共价闭合环状DNA（CCC－DNA）。

DNA gyrase is an essential bacterial topoisomerase that catalyzes the ATP dependent negative supercoiling of double stranded closed circular DNA .

DNA回旋酶是细菌特有的一种拓扑异构酶，它能够利用ATP水解的能量将共价闭合环状DNA转变为负超螺旋DNA，从而维持细菌体内正常的超螺旋水平。

[ Objective ] To study the clinical significance of serum hepatitis B virus covalently closed circular DNA ( HBV cccDNA ) determination in patients with chronic HBV infection .

探讨慢性乙型肝炎病毒感染者外周血乙型肝炎病毒共价闭合环状DNA检测的临床意义。

Objective To study the effect of hepatitis B virus ( HBV ) C protein C-terminal small interference RNA ( siRNA ) on reduction of HBV covalently closed circular DNA ( cccDNA ) level .

目的研究乙型肝炎病毒（HBV）C蛋白羧基端短小的干扰RNA（siRNA）对HBV超螺旋双链闭环DNA（cccDNA）水平的影响。

The pBR322 recombinant plasmids DNA products , which purified by phenol-extract and equilibrium centrifugation in CsCl - ethidium bromide gradients , have been shown as mainly spatial conformation of covalently closed circular DNA ( i. e. cccDNA ) by agarose gel electrophoresis for homogeneity detection .

将pBR322重组质粒DNA纯化，经琼脂糖凝胶电泳鉴定其主要具共价闭合环状空间构象。

The genome is a double stranded , covalently closed , circular , supercoiled DNA molecule , approximately 8000 base pairs in size .

基因组为闭合环状双链超螺旋DNA，由大约8000对碱基组成；