chain termination

The chain termination is second order with respect to the concentration of active species .

链终止反应速度与活性中心浓度的二次方成正比。

Evaluation of chain termination in radical polymerization based on the molecular weights of polymers

从聚合物分子量评价自由基聚合的链终止反应

Sequencing of HBV ( adr ) DNA Restrictive Fragment by Dideoxy Chain Termination Method

末端终止法测定HBV（adr亚型）DNA酶解片断序列

Initiation efficiency was 0.11 . Chain termination rate constant kt was calculated with the experiment data of radical concentration R.

引发效率为0.11。

The amplified product with 238 bp was purified and then directly sequenced by dideoxy nucleotide chain termination method .

PCR产物纯化后直接经双脱氧链末端终止法测定核苷酸序列。

De termination of DNA Sequence with Chain Termination Method and Labeling by α: - ~ ( 32 ) p-dTTP

α~（32）P-dTTP标记，用末端终止法测定核苷酸排列顺序

Dideoxy chain termination method was applied to sequence hepatitis E virus structure gene of Asia isolates , cDNA sequence and amino acid residues were determined .

目的了解戊型肝炎病毒的变异。方法用双脱氧法对亚洲主要戊型肝炎流行国家的戊型肝炎病毒结构基因区段进行了分析，并根据cDNA序列确定了其氨基酸的序列。

The DNA sequences of RH-2 and RH-3 were confirmed by the dideoxynucleotide chain termination method .

DNA序列测定确认了所构建得的RH-2和RH-3基因。

The Mechanism of Chain Termination in the Cationic Polymerization of IBVE Initiated by Cl_2 / TiCl_4 / CH_2Cl_2 System

IBVE/Cl2/TiCl4/CH2Cl2体系阳离子聚合链转移终止机理的研究

Methods : the sequences of the full viral genome were determined by DNA chain termination method and then the phylogenic tree was completed by DNA - STAR software .

方法：在对肠道病毒71型中国（深圳）分离株SHZH03进行全基因组序列测定的基础上，利用DNA-STAR软件对VP2和VP4基因进行遗传进化分析。

In addition , Sanger method ( dideoxy mediated chain termination method ) of DNA sequencing was used to examine the exon 7 which was positive in p53 mutation .

运用双脱氧链终止法对第7外显子阳性突变标本进行DNA测序。

The positive recombinant plasmid was identified by PCR , enzyme digestion . The nucleotide sequence of CSP3 ′ ending gene was determined by the dideoxy chain termination method .

阳性重组质粒经PCR、酶切鉴定，用双脱氧链末端终止法进行序列测定。

As radical polymerization solvent , ionic liquids could increase the propagation rate constant kp and reduce the chain termination rate constant kt , and higher yield and higher molecular weight were obtained as well .

离子液体作为自由基聚合的溶剂时，可以提高链增长速率常数，降低链终止速率常数，并获得更高的产率和产物分子量。

The cDNA encoding the extracellular domain of human costimulatory molecule B7 1 / CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method .

用RTPCR法从人外周血单核细胞克隆了编码共刺激分子B71/CD80胞外区的cDNA，并用Sanger链终止法进行测序。

Lamivudine inhibits viral replication at the level of progeny DNA synthesis . Specifically , its incorporation into nascent DNA causes chain termination . This applies both to the reverse transcriptase-and the DNA-dependent DNA polymerase activity of the virus .

Lamivudine是一种目前广泛应用的抗病毒药物，它可同时抑制反转录酶和DNA依赖性DNA聚合酶的活性，从而同时阻断HBV负链和正链DNA的合成。

Methods The LDH gene was amplified by PCR technique from genomic DNA of isolate FCC 1 / HN . Then it was cloned into pMD-18T vector . The cloned LDH gene was deter - mined by the dideoxy chain termination method .

方法根据LDH基困已知序列设计合成一对引物，应用PCR技术从FCC1／HN株基因组DNA中扩增LDH基因，并将其克隆入pMD18－T载体。

The M RNA segment of Hantaan virus Chinese isolate A9 was reverse-transcribed into the first strand of cDNA and amplified into cDNA fragments which were cloned into pGEM-T vector . The positive clones were sequenced on double strand level by dideoxy chain termination method .

采用反转录-PCR方法，分节段扩增汉坦病毒A9株M基因片段cDNA，并克隆入PGEMT载体中，用双脱氧末端终止法直接测定序列。

The sequencing of the PCR product was determined by using dideoxy - mediated chain - termination method .

采用双脱氧DNA链合成终止法进行PCR产物直接测序，确认基因型的测定结果。

It was found that the chain initiation and termination reactions were dependent on the species of the block chain active centres and only the lower active species . could initiate α es while the higher active species were terminated rapidly by α MS.

结果表明，链引发和链终止反应与嵌段链活性中心的形态有关，只有嵌段链活性中心的低活性形态才能同αMS发生引发反应，而高活性形态则与αMS发生链终止反应。

Methods The RNA ( nt1460 1582 , HCV J6 ) extracted from plasma of 38 patients infected with genotype III / 2a HCV were transcribed , amplified , purified and directly sequenced by using RT nested polymerase chain reaction ( PCR ) and dideoxynucleotide chain termination method , respectively .

方法应用逆转录巢式PCR技术，从38例III/2a型HCV感染患者血清中，扩增HCV部分包膜区基因片断（nt1460～1582，HCVJ6），纯化后直接采用双脱氧链末端终止法进行序列分析。