calb

The enzymatic characteristics of the recombinant CALB were also determined .

对重组CALB进行了初步的酶学性质分析。

In the enzyme property assay of CALB displayed on Pichia yeast .

对毕赤酵母表面展示CALB全细胞催化剂的酶学特性进行研究。

Purification of CALB by Ammonium Sulphate-Actone Concerted Precipitation Method

硫酸铵-丙酮协同沉淀法纯化南极假丝酵母产脂肪酶

It has a strong catalytic activity in non-water-soluble and water-soluble systems . CALB shows greater superiority than other lipases in a variety of reactions because of its stability and specificity .

它对于非水溶性和水溶性物质都有很强的催化活力，并且具有良好的稳定性，在转酯等反应中显示出优于其他脂肪酶的性质。

The work consists of three parts : ( 1 ) Investigating the conformation changes of Candida antarctica lipase B ( CALB ) in several polar and non-polar organic solvents by molecular dynamics simulation .

研究分为三部分：（1）通过分子动力学模拟方法研究南极甲丝酵母脂肪酶B在极性有机溶剂和非极性有机溶剂中的构象活性变化规律。

For its broad spectrum of substrate acceptance and enantioselectivity , Candida antarctica lipase B ( CALB ) has been widely used in the fields of food , chemicals , drug precursors synthesis and bio-energy .

南极假丝酵母脂肪酶B（CandidaAntarcticalipaseB，CALB）具有广谱底物接受性和对映体选择性，广泛应用于食品、化工、前体药物的合成和生物能源等领域。

CALB was purified through Ni-NTA affinity chromatography and ultrafiltration , and was then analyzed by SDS-PAGE . The result showed that the CALB protein expressed by P. pastoris was about 35 kDa , which might contain the glycosylated brach chain .

对经Ni-NTA亲和层析和超滤浓缩纯化的CALB进行SDS-PAGE分析，发现在CalB基因在P.pastoris中表达产物的大小为35kDa（含糖基化侧链）。