The envelope gene of ADOL 4817 strain of avian leukosis virus subgroup J ( ALV J ) was amplified by polymerase chain reaction ( PCR ) and cloned into TA vector .
应用多聚酶链反应（PCR）的方法扩增出ADOL4817毒株的囊膜蛋白env基因，并克隆进大肠杆菌。
adol