X-Gal

Methods : Determine the efficiency of adenovirus-mediated gene transduction by X-gal stain .

目的：观察携动物毒素基因重组腺病毒在体外对人肝癌细胞的杀伤作用。方法：用X-gal染色法测定腺病毒介导基因转染的效率；

The infection efficiency of adenovirus vector to MSCs was tested by x-gal stain .

用x-gal染色测含肾上腺髓质素基因的重组腺病毒（Ad-ADM）对骨髓间充质干细胞感染率。

The recombinant clone was screened by X-gal plate and plasmid map , and identified by restricted enzyme mapping analysis method .

用X-gal平板及质粒图谱分析方法筛选重组克隆株，再用限制性核酸内切酶酶切图谱分析鉴定。

The difference between the effects of lactose and X-gal on the conformation of AAL is also showed by the synchronous spectrum .

用同步荧光光谱法比较了乳糖和X-gal对AAL构象的影响，结果表明，乳糖和X-gal对AAL的结合方式有可能不同。

The efficiency of recombination reached to 100 % by the color selection on the a-garose plate containing IPTG and X-Gal .

经含有IPTG及X－Gal的颜色选择平皿测定，重组效率为100%。

Mice embryos were transfected with Ad vectors and detected the infection efficiency by X-Gal stain to determine the exact MOI for human embryo transfection .

通过X-Gal染色法检测重组腺病毒载体感染小鼠胚胎的效率并确定最佳MOI值。

The X-gal blue staining rate of transduced cells was about 13.33 ± 2.68 % in human and about 16.28 ± 2.95 % in mouse without G418 selection .

其蓝染阳性细胞率在人及小鼠造血细胞于G418选择前分别为13.33±2.68%和16.28±2.95%。

The bacterio-liquid was spreaded on the plate culture medium contained the Aminobenzylpenicillin , the X-gal and IPTG . ⑦ Then , we picked out the needed germs by drug-resistance screening and the blue-white spots test .

将培养好的菌液平铺于含有抗生素和X-gal、IPTG的平板培养基中，通过抗药性筛选及蓝白斑试验挑选所需的大肠杆菌，继续于液体培养基中过夜培养。

At last , protein interaction could be identified by X-Gal verification . The results showed that the recombinant bait plasmid and capture plasmids were constructed successfully . The target gene could be expressed in yeast cells . There was no self-activation and toxicity for yeast .

结果表明，重组诱饵质粒与捕获质粒均构建成功，目的基因可以在感受态酵母细胞中表达，诱饵蛋白对酵母细胞既没有毒性，也不存在自激活作用。