Wright staining

Wright staining shows that the mature T cells harvested after mitogenic stimulation have the presence of large cells with lymphoblast morphology .

培养成熟的T淋巴细胞经PHA+IL2刺激后瑞氏染色鉴定可见大原始淋巴细胞存在。

In order to examine the endothelialization of yak pericardial materials in vitro , we used Wright staining and successfully observed the morphology , number and order of endothelial cells seeded on pericardial patches under light microscope .

在牦牛心包生物心瓣膜材料体外内皮化实验中，采用瑞氏染色法，成功地在普通光镜下观察到了心包片上种植的血管内皮细胞形态、多少及排列状况。

METHODS : The number of eosinophils in the blood and BALF were counted with Wright staining .

方法：采用Wright染色法计血液和BALF中Eos的数目。

Differentiation changes of the K562 cells were observed by Wright staining , flow cytometry and benzidine staining .

采用Wright染色、流式细胞术、联苯胺染色观察K562细胞的功能变化，以评价L1对K562细胞的诱导分化作用。

Methods : The number of eosinophilias in the blood and BALF were counted with Wright staining .

方法：用Wright染色法计血液和肺泡灌洗液（BALF）中嗜酸性粒细胞（EOS）的数目。

Cells were harvested at 72 hours later , their growth was observed with Wright staining , LDH cytochemistry and immunocytochemistry .

将巨噬细胞培养上清加入低浓度血清培养的成肌细胞中，作用72h后收集细胞，通过Wright染色、LDH细胞化学和免疫细胞化学方法观察成肌细胞的生长情况。

All cells and small tissues were planted in 12-well culture plate and the culture cells morphological characteristics were observed by Wright staining during the various time periods .

细胞接种于12孔培养板，并于培养不同时间行Wright染色检查细胞生长情况。

Bcap-37 cells were treated by different concentration of FHR at diverse time periods , which was identified by MTT , Wright staining , flow cytometry and immunohistochemical methods .

采用MTT法、Wright染色法、流式细胞术及免疫细胞化学等方法，观察在不同浓度和作用时间时，FHR对Bcap-37细胞生长抑制和细胞凋亡效应。

After being incubated for 48 h the supernatant was harvested for determination of IL-1 β and TNF - α concentrations . The morphological changes of the cells were examined by Wright staining . The apoptotic neurons and astrocytes were detected by flow cytometry .

培养48h后取各组细胞上清液检测白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）浓度，瑞氏染色观察细胞形态变化，流式细胞仪检测神经元和星形胶质细胞凋亡。

Results There was no significant difference between Wright 's staining craft classification and instrumental method ;

结果两台仪器的白细胞分类结果与手工分类法无显著性差异。

The cell apoptosis was detected by flow cytometry , and the cell morphology was observed by Wright 's-Giemsa staining .

瑞氏-吉姆萨染色观察细胞形态学变化、流式细胞术测定细胞凋亡率；

Femur bone morrow slides were made by Wright 's staining and qualitative and quantitative observation on bone marrow image .

采用姬姆萨-瑞氏染色法制作股骨骨髓涂片，对骨髓象进行定性、定量观察；

Methods Wright 's staining coated pieces with puncturing marrow of 98 cases were tested by oil lens of OLYMPUS microscope made in Japan .

方法瑞特染色用日本产的显微镜对98例患者的骨髓穿刺涂片进行油镜检查。

Methods 160 samples with abnormal parameter hinted by the analyzer and 112 samples without hinted abnormal parameter were tested comparative with microscopic examination of blood smears after Wright 's staining .

方法将160例仪器提示异常参数的标本和112例无异常提示的标本作血涂片，经瑞特-姬姆萨染色镜检后比较结果。

Hyaline leucocyte with the size of 5.63 ± 1.49 μ m possesses a heavily stained nucleus but no granules in the cytoplasm when treated by wright 's staining ;

透明细胞为5.63±1.49μm，核染色深，瑞氏染色胞质内光镜下未见颗粒；

Methods 200 normal and 104 abnormal specimens which were detected by cytoanalyzer were stained with Wright 's staining , then the cells'morphological examination and number counting were rechecked by ordinary microscope .

方法随机抽取用全自动血细胞分析仪分析为正常的200份和异常的104份标本，涂片瑞氏染色作显微镜血细胞形态观察及手工计数血细胞等方法进行复检。

Methods : Apoptotic spermatogenic cells were examined by Wright Giemsa staining and terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate ( dUTP ) biotin nick end labeling ( TUNEL ) technique .

方法：采用瑞－姬染色和末端脱氧核苷酸转移酶（TdT）介导的脱氧核苷酸原位末端标记法（TUNEL）检测凋亡生精细胞。

Two hours after the last medicine , the blood sample was adopted by cutting the mice tails , and Wright 's staining and immersion objective were used to count the percent of the transformation efficiency of lymphocyte in peripheral blood .

于最后一次给药后2h，小鼠剪尾取血，瑞氏染色，油镜观察并计算外周淋巴细胞转化百分率。

Methods : NRBCs were isolated from 6ml of peripheral blood with density gradient centrifugation from 236 women with 28 ~ 42 weeks ' gestation . Blood smear slides were prepared for Wright 's staining and cell counting .

方法：取236例28～42周孕妇外周血各6ml，密度梯度离心分离胎儿NRBC，制片、瑞氏染色、细胞计数；

Conclusion Wright Giemsa compound staining is easy and quick , with bright and clear specimens , which is worth of clinical application and teaching .

结论吉姆萨瑞特染色法操作简便、快速，卡氏肺孢子虫标本着色清晰，是临床诊断和教学中值得推广和应用的染色方法。

Methods On the basis of a general survey by clinic and X - ray , peripheral bloods and bone marrow aspirates of the diseased children were sampled , smeared , stained ( Wright - Giemsa staining ) and observed with a light microscope .

方法在临床X线普查基础上，采病儿外周血及骨髓吸出物涂片、Wright－Giemsa染色、光镜观察。