Wright stain

Methods : Radioimmunoassay ( RIA ) and Wright Giemsa stain were used to detect serum sexual hormone and sperm cytology in 45 aspermia patients .

方法：45例无精子症病人采用放射免疫法（RIA）检测性激素，瑞吉染色法进行精液细胞学检查。

0.5 mL solution obtained from abdominal cavity was cultured at 37 ℃ for 30 minutes , fixated with methanol and stained with Wright 's stain .

取腹腔洗液0.5mL滴片，37℃温育30min，甲醇固定，瑞氏染液染色。

Metbod : Effects of STT on the cytotoxicity , morphological changes of apoptosis , cell cycle and expression of Bcl-2 protein in the 786-O were tested respectively by MTT method , Wright and acridine orange stain assay , as well as flow cytometry ( FCM ) .

方法：应用MTT法、Wright染色及AO荧光染色法和流式细胞术（FCM）及结合免疫荧光分别测定STT对细胞的生长抑制作用，细胞凋亡的形态变化，细胞周期及Bcl-2蛋白表达的影响。