VTG

Studies on the Correlation between Serum VTG and Serum Calcium of Juvenile Carassius auratus after Exposure to E_2

E2诱导的鲫鱼（Carassiusauratus）幼鱼血清中卵黄蛋白原和钙含量相关性研究

VTG mRNA in male fish is a new kind of biomarker .

雄性鱼类VTGmRNA是一种检测环境雌激素的新兴生物标志物。

The expression of VTG in BPA treated group is less than E2 treated group .

BPA处理组的VTG表达量低于E2对照组。

Due to the increasing pollution in marine environment , Vtg of marine fish receives great concern .

随着环境污染的加剧，大量污染物被排入大海，使沿海污染日益严重，因此有必要开展海洋鱼类Vtg的研究，用于监测海洋环境雌激素的污染。

In BPA treated group , the expression of VTG is increased with the increasing treated time and increase BPA .

在BPA处理组中，VTG表达量随BPA处理时间的延长而上调，随处理浓度的升高而上调。

Vtg had several subunits . The molecular weight of the major subunit is 110 kD .

Vtg的分子量约为556.5kD，主要亚基大小为110kD。

The results showed that the Vtg concentration in male loach serum were significantly increased with the increase of exposure time and dose .

结果表明，雄性泥鳅血清Vtg含量随BPA暴露时间和暴露浓度的增加而显著增加。

Studies so far about Vtg as a biomarker for environmental estrogens were mostly limited to freshwater fish .

目前对Vtg的研究主要集中在淡水鱼类，对海水鱼类的研究较少。

Vitellogenin ( VTG ) was measured with ELISA kit , in transgenic carp and wild carp .

卵黄蛋白原（VTG）测定用酶联免疫反应法，检测转入生长激素的转基因鲤鱼和亲本鲤鱼的VTG水平。

It is hopeful to reduce the content of egg cholesterol by modifying the gene expression of apo-II and VTG .

可望通过调控apo-II和VTG的基因表达来降低蛋黄胆固醇含量。

An immunochromatographic test strip based on gold colloid was developed to quickly identify vitellogenin ( VTG ) induction in fish in field waters .

利用免疫层析和胶体金标记技术试制出一种免疫检测试片，该试片可快速定性检测鱼体内诱导的卵黄蛋白原。

Environmental estrogens screening methods , with VTG mRNA as a biomarker , include real-time quantitative PCR , Northern blot , in situ hybridization .

以VTGmRNA为生物标志物的环境雌激素筛选方法主要包括实时定量PCR、Northern杂交、原位杂交等。

Although there has been several researches proving that fish meal with genistein could induce VTG synthesis in juvenile and male fish , the mechanisms is still unknown .

目前，虽然有研究证实含金雀异黄素的饵料喂养能诱导雄鱼及幼鱼体内VTG的产生，但对其诱导机理却尚未进行深入研究。

The expression of VTG and BPA is the dose-time-response relationship . In E2 treated group , the expression of ER is change in a similar trend .

表明VTG的表达与BPA间有剂量-时间效应关系，与E2处理组的效应相似。

The relative estrogenic effects of several phenolic compounds were evaluated by using VTG of juvenile goldfish ( Carassius auratus ) as a biomarker .

采用金鱼（Carassiusauratus）幼鱼的卵黄蛋白原（VTG）作为雌激素污染的生物标志物，评价了几种酚类化合物的雌激素效应。

Conclusion : ATZ can disturb endocrine system , induce estrogen effect , increase gene expression of VTG , and presents a kind of monotonous concentration effect relationship at low concentration .

因此本研究认为：（1）ATZ能够对内分泌产生干扰，在低浓度时诱导雌激素效应，上调VTG基因的表达，并呈现一种非单调的浓度效应关系。

The results demonstrated that there was good dose-related response between the concentration of E_2 and serum VTG as well as serum calcium of juvenile Carassius auratus .

结果表明：鲫鱼幼鱼血清中VTG和钙含量与E2的暴露剂量均有很好的剂量-效应关系。

Based on the analysis of the dynamic distortion camouflage technology , the calculation was offered as for the VTG 120 thermal imaging guidance head equipped by the TOW anti-tank missile .

本文在介绍动态变形伪装原理的基础上，以TOW反坦克导弹携带的VTG120热成像导引头为例进行了计算分析，结果表明，动态变形伪装能够使坦克等目标的生存概率大大提高。

Results Compared with control group , body weight and body length of zebrafish decreased ( P < 0.01 ) and its vtg concentration increased ( P < 0.01 ) after EE_2 treatment .

结果与对照组相比，EE2组斑马鱼的体重、体长降低，P<0.01；VTG含量明显增高，P<0.01；

An indirect competitive enzyme-linked immunosorbent assay ( ELISA ) for detecting Vtg content in the blood of male Carassius auratus was developed with antiserum of lipovitellin resistance as an .

以抗卵黄脂磷蛋白抗血清作为抗体，卵黄蛋白原作为竞争抗原建立了间接竞争法酶联免疫吸附反应（ELISA）方法来检测雄鱼血液中卵黄蛋白原含量。

Objective To study the effect of ethynylestradiol ( EE_2 ) on vitellogenin ( vtg ) and gonad development of zebrafish ; to select sensitive biomarkers for screening environmental estrogens using zebrafish .

目的研究炔雌醇（EE2）对斑马鱼的卵黄蛋白原（VTG）及性腺发育的影响，并通过一系列生殖发育终点指标的研究选择有效筛选环境雌激素（EEs）的敏感指标。

Using degenerate primers Rare Minnow VTG and part of p53 gene fragments , and contrasting entire gene sequence with zebra fish and other species of fish found the Similarity was in 93 % and 80 % .

利用兼并引物扩增出稀有鮈鲫VTG和p53基因的部分片段，与斑马鱼等多种鱼的全基因序列比对发现相似度分别在93%和80%以上。

The major findings are as follows : ( 1 ) Single EDCs impacted morphology of zebrafish embryos . Expression of VTG gene was increased because of estrogen inducing . Expression of ER α gene was inhibited by fulvestrant .

主要研究结果如下：（1）单一EDCs作用对斑马鱼胚胎发育形态学有不同程度的影响；VTG基因被雌激素诱导表达量增加，ERα基因表达被抗雌激素氟维司群所抑制。

Egg yolk cholesterol mainly come from yolk precursor-very low density lipoprotein ( VLDL ) and vitelline ( VTG ) . The content of egg yolk cholesterol depends on the amount of VLDL transported into oocyte .

蛋黄胆固醇主要来源于肝脏合成的蛋黄前体&极低密度脂蛋白（VLDL）和卵黄蛋白原（VTG），蛋黄胆固醇含量主要取决于VLDL进入卵母细胞的数量。

It can be concluded that VTG expression in zebrafish embryos can also be served as a biomarker to analyze and assess endocrine disrupting of compounds . Zebrafish embryos can be used as a model organism to carry out the estrogenic effects of endocrine disrupting chemicals .

因此，通过检测斑马鱼胚胎中VTG基因的表达，同样可作为一种生物标志物，分析评估化合物的内分泌干扰效应，斑马鱼胚胎可以作为一种模式动物开展多种内分泌干扰物的类雌激素效应研究。

Vitellogenin ( VTG ) was induced in red drum by intraperitoneal injections of 17 β - estradiol . After one week induction , blood was collected from caudal vessel and then centrifuged . Purification of the VTG was performed by gel permeation chromatography of Sephacryl S-300 high resolution .

腹腔注射0.02mg/gBW17β-雌二醇，诱导美国红鱼1wk后取尾静脉血，离心分离得到的血浆经Sephacryls-300highresolution分子筛分离、纯化卵黄原蛋白。