Tris-HCl

Methods Histone , SDS and DNA were mixed into the Tris-HCl buffer solution ( pH 7.5 ) .

方法pH7.5的Tris-HCl缓冲液体系中，将组蛋白与DNA及SDS混合。

Its optimum pH was 8.8 in boracic acid buffer , and 8.0,8.8 in Tris-HCl buffer .

PAL在硼酸缓冲液中最适pH是8.8，在Tris-HCl缓冲液中最适pH为：pH8.0、pH8.8。

Different amount of Tris-HCl buffer were solubilized in the reverse micelles made from heptane-butanol-lecithin .

以正庚烷-正丁醇-大豆卵磷脂配制反胶束，做了增溶不同量Tris-HCl缓冲液的试验。

Weight loss of electrospun membrane was 39.09 % after 10 hours of degradation in Tris-HCl / Proteinase K buffer solution .

在Tris-HCl/ProteinaseK缓冲液中降解10小时后，电纺膜减重率达到了39.09%。

Methods : We improved the traditional method of extract DNA by using the mixture of Triton-X and Tris-HCl to lyses RBC .

方法改良经典基因组DNA提取方法，用TritonX-100和Tris-HCl混合溶解红细胞。

Results : In comparison of the two methods for extracting DNA , Tris-HCl was found to be simpler , though they showed no significant differences in DNA concentrations and quality .

结果：比较两种方法抽提DNA，发现Tris-HCl法与Chelex-100法抽提DNA在浓度和质量无明显差异，Chelex-100法步骤简便。

The influence of H_3BO_3-NaOH , Tris-HCl and Na_2HPO_4 buffers on the performance of apple tissue-based sensor has been studied , the sensor displayed optimum response properties in H_3BO_3-NaOH buffer .

系统地研究了H3BO3-NaOH、Tris-HCl及Na2HPO4三种缓冲体系对苹果组织传感器性能的影响，在H3BO3-NaOH缓冲液中苹果组织织传感器有最佳性能。

The results of cell culture : The Sertoli cells survival rate above 95 % . After 24 hour the cells began to grow and 48 hours to spread , which purification more than 90 % be treatmented by Tris-HCl . 2 .

结果1细胞培养结果：细胞存活率在95%以上，24小时开始贴壁生长，48小时以后开始铺开生长，经Tris-HCl低渗液纯化后Sertoli细胞纯度在90%以上，细胞生长良好。

The results show that Thanh is the best method for extracting 11S globulin from soybean ; the appropriate solvent is Tris-HCl buffer ; the optimum extraction condition are time 2h , material / buffer 1 ∶ 20 , and temperature 25 ℃ .

结果表明，Thanh法提取分离大豆11S球蛋白效果较好，Tris-HCl缓冲液是适宜的提取液，最优提取工艺条件为提取时间2h、料液比1∶20、提取温度25℃。

The substances were precipitated using saturated ammonium sulfate solution , redissolved in Tris-HCl buffer , and then filtered using a 100 kDa ultrafiltration cube , the results showed that the active components of the substance was trapped by the ultrafitration cube .

该活性物质经硫酸铵盐析沉淀，沉淀复溶于Tris-HCl缓冲液后，使用100KDa超滤管进行超滤，该物质被截留，说明其分子量大于100KDa。

The stability of Acid protease produced by Aspergillus niger was studied , including heat stability , acid stability , metals ion stability , etc . VacA-IgY was added into Tris-HCl of different pH values and incubated under a temperature of 37 ℃ to access its acid stability using ELISA .

通过黑曲霉固体发酵生产酸性蛋白酶，对此酸性蛋白酶的部分酶学稳定性（耐热、耐酸性、金属离子稳定性等）进行实验探讨。在不同pH的Tris-HCI中孵育一定时间，评价其耐酸性；

In the Tris-HCl buffer solution at pH 7.0 , REs can react with TAAP to form a stable 1 ∶ 3 red complex , which maximum absorption wavelength is located at 523 nm . Beer 's law is obeyed in the range of 0.07-1.5 μ g / mL.

在pH7.0的Tris-HCl缓冲溶液中，REs与TAAP生成组成比为1∶3的稳定红色络合物，其最大吸收波长位于523nm处，REs总量在0.07~1.5μg/mL范围内符合比尔定律。

When tea pigments were washed with Tris-HCl buffer ( pH 8.0 ), the washing efficiency was the highest in first washing and the contents of TFs increased with washing times , but the volume of washed liquid was decreased accordingly , and there was de-galloyl group action during washing .

对茶色素进行TrisHCl（pH80）洗涤，其洗涤效率也以第1次最高，TFs含量随洗涤次数增多呈现递增趋势，但被洗液体积呈递减趋势，并存在脱没食子酰基作用。