T7 RNA Polymerase

A method of siRNA synthesis by T7 RNA polymerase transcription

T7RNA聚合酶体外转录合成siRNA的方法

Objective : To establish a method using T7 RNA polymerase for obtaining large amount of siRNA .

目的：建立一种应用T7RNA聚合酶体外转录合成大量siRNA的方法。

Cloning and Sequencing of T7 RNA Polymerase and Its Expression in E.coli

T7RNA多聚酶基因的克隆、序列测定及其在E.coli中的表达

Expression of Cecropin A Genes by Bacteriophage T7 RNA Polymerase

用细菌噬菌体T7RNA聚合酶高效表达天蚕蛾菌素（cecropinA）基因

Objective To synthesize highly pure HBV post-transcriptional regulatory element ( HPRE ) via transcription in vitro by T7 RNA polymerase .

目的探索T7RNA聚合酶体外转录方法制备乙型肝炎病毒（HBV）转录后调节基序（HPRE）。

However , the expression of T7 RNA polymerase usually depends on the vaccinia vector , leading to inevitable contamination of viruses in the preparations .

但是，T7RNA聚合酶自身的表达往往依赖重组痘苗病毒载体，导致产物中含有没有去除的痘苗病毒。

In this study , we assemble a positive feedback loop for the production of T7 RNA polymerase using basic molecular elements , including CMV promoter , T7 promoter and the internal ribosomal entry site ( IRES ) .

在本课题中，利用基本的分子生物学元件，如CMV启动子、T7启动子、和内部核糖体进入位点（IRES）等，我们组装了一个T7RNA聚合酶的正反馈表达系统。

In this paper , we have developed several detection methods for small molecules-protein interaction and copy number variations based on nucleic acid amplification methods as follows : ( 1 ) We developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex .

本文发展了几种检测小分子-蛋白质相互作用以及拷贝数变异的核酸扩增检测方法：（1）结合T7聚合酶与一个小分子标记的DNA双链，发展了一种RNA转录器件。

Methods The mouse caspase-12 gene fragment was obtained by RT-PCR and cloned into the PGEM-T vector under the control of T7 RNA polymerase promoter . The transcription product of the target was labeled with α - 32P UTP , while ribozymes were not labeled .

方法小鼠Caspase-12基因的逆转录聚合酶链反应（RT-PCR）扩增片段克隆于PGEM-T载体的T7启动子下游，通过α-32PUTP标记的体外转录物作为靶RNA。

In the end , by means of the T7 promoter in the pBlueskript ⅱ KS ( + ) and T7 RNA polymerase , DIG-labelled PS gene full-length and specific fragment RNA anti-sense probe was synthesized by in vitro transcription .

最后利用pBlueskriptⅡKS（+）上的启动子T7，用T7RNA聚合酶在体外转录合成地高辛标记PS全长及特异序列的反义RNA探针。