Stable transfection

After stable transfection , the cell strain with the highest expression of target protein was served as engineering cell strain .

稳定转染后选取表达量最高的细胞株为工程细胞株。

Methods Human ER α cDNA was reintroduced into MDA-MB-435 cells by stable transfection .

方法：通过基因稳定转染技术，构建ERα稳定表达的MDA-MB-435细胞株。

The establishment of stable transfection of human gallbladder cancer cell line with exogenous wild-type p53 gene

稳定表达外源野生型p53基因人胆囊癌细胞系的建立和鉴定

Continuous expression of green fluorescent protein suggests that the vector is possible to mediate stable transfection of DNA .

荧光蛋白的持续表达提示其可以介导DNA稳定转染。

Stable transfection with antisense survivin inhibited cell growth , proliferation , and promoted cell differentiation and apoptosis .

反义survivin抑制胶质瘤细胞株SHG-44细胞的增殖、促进其分化和凋亡。

Methods The ER negative breast cancer cell line MDA MB 231 was transfected with the ER gene by stable transfection .

方法雌激素受体阴性的乳腺癌细胞株MDA－MB－231细胞，采用分子生物学质粒转染技术，将ER阴性的乳腺癌细胞MDA－MD－231转染成ER阳性细胞。

Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used .

方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。

The stable transfection and expression level of ANG in TE-1 were determined by semi-quantitative RT-PCR and Western blot .

半定量RT-PCR法及蛋白质印迹法分别观察TE-1细胞中ANG在核酸和蛋白水平表达量改变。

AIM : To observe the possibility and implication of stable transfection of full length HAb18G cDNA into NIH / 3T3 cell line .

目的：探讨将HAb18G全长cDNA转染成纤维细胞NIH/3T3的可行性及其意义。

Objective : To study the condition of electroporation on human nasopharyngeal carcinoma cells ( NPC ) and to get a cell model of stable transfection by EBV-LMP gene .

目的：观察人鼻咽癌细胞用电穿孔技术进行体外基因转染所需的转染电击条件及EBV－LMP基因的转染表达。

Objective To investigate the effects of stable transfection of interferon β ( IFN - β) gene on the apoptosis of human gliomas cells in order to explore new methods of gene therapy for glioma .

目的研究外源性干扰素-β（IFN-β）基因对胶质瘤细胞系SHG-44的诱导凋亡作用，探索胶质瘤基因治疗的新途径。

Objective To investigate the feasibility of stable transfection of human hypoxia inducible factor-1 α( HIF-1 α) gene into fibroblasts cells and the effects of supernatant from the transfected cell culture on hair follicle cells .

目的探讨外源性人低氧诱导因子-1α（HIF-1α）在成纤维细胞中表达的可行性及其对毛囊周围细胞活性的影响。

Results : Transient transfection efficiency was 30 % ~ 40 % , while stable transfection efficiency was about 1 / 10 4 ~ 5 . Furthermore , all stably transfected human embryonic stem cells expressed EGFP .

结果：EGFP瞬时转染效率为30%～40%，稳定转染效率约1104～5，且稳定转染的人胚胎干细胞均表达EGFP。

Methods : To determine the function and binding properties of SR-A in HMC , human mesangial cell line ( HMCL ) with high expression of SR-A ( HMCL-SR-A ) was established after stable transfection of expressive vector with cDNA encoding SR-A.

方法：（1）脂质体转染含A型清道夫受体cDNA的表达质粒，建立稳定高表达A型清道夫受体的人肾小球系膜细胞株（HMCL），分析清道夫受体的功能和受体特异性；

Gene therapy for the treatment of these patients with serious coronary artery disease can provide a new treatment strategy , since it still lacked a safe , stable and efficient gene transfection method which can limit gene therapy in a wide range of clinical applications .

近年来基因治疗迅速发展，为冠心病患者提供了一个新的治疗策略，但目前由于尚缺乏一种安全、稳定、高效的基因转染方法，而极大地限制了基因治疗在临床上的广泛应用。