Solanum dulcamara

Methods : The human lung cancer cell line SPC-A-1 was in vitro co-cultured with solanum dulcamara at 4 different concentrations for 24,48,72 and 96 h ;

方法：选用人肺腺癌SPC-A-1细胞株为研究对象，在体外与白英提取物4种不同浓度共同培养24、48、72、96h，采用MTT法检测白英提取物对SPC-A-1细胞增殖活性的影响；

Results : The solanum dulcamara water extract inhibited proliferation of SPC-A-1 cells , and an apoptotic ladder was detected using AGE .

结果：白英提取物抑制SPC-A-1细胞增殖活性；

Conclusion : A specific concentration of solanum dulcamara water extract can inhibit SPC-A-1 cell proliferation and can induce apoptosis of SPC-A-1 cells . The mechanism of action is related to expression of the bcl-2 gene .

结论：白英水提取物对人肺腺癌SPC-A-1细胞株有抑制增殖、诱导其凋亡作用，细胞凋亡的机制可能与细胞凋亡相关基因Bcl-2表达下调有关。

The expression of the apoptosis related gene bcl-2 after treatment with 50.0 mg / ml solanum dulcamara extract for 48h ( 24.41 % ± 2.96 ) was lower compared to untreated cells ( 53.53 % ± 4.80 )( P < 0.001 ) .

50.0mg/ml白英提取物处理48h后的细胞凋亡相关基因Bcl-2表达率（24.41±2.96）%比未经处理的细胞（53.53±4.80）%表达率低（P<0.001）。