Secondary Culture

The results showed that The best secondary culture prescription for Pomegranate was : MS + 1.0mg/L2 , 4-D + 0.1 mg / LKT .

结果表明石榴最佳继代培养配方为：MS+1.0mg/L2,4-D+0.1mg/LKT。

[ Methods ] : RLECs were primary culture and secondary culture .

方法：取正常兔晶状体上皮细胞（RLECs）进行原代培养及传代培养。

The primary and secondary culture of the fibroblasts in tissues were proceeded with the cell culture technique , while being purified .

⑶实验三：将原代培养的肛瘘瘘管组织细胞进行酶消化法纯化培养获得上皮细胞，采用免疫细胞化学进行细胞起源鉴定。

Seed volume in factory production was that 3 ~ 5g / 50ml in a seed culture tank for 2 weeks , 30g / 400ml in a secondary culture tank for 2 weeks and then transferred into productive tank till harvest .

发根工厂化培养适宜的接种量：种子罐为3～5g/50mL，培养2周，次级罐接种量为30g/400mL，再培养2周后，即可转入生产罐（5L）中继续培养至采收。

Modern economic development need vocational secondary academies culture more professional person for society .

经济的发展需要中等职业学校为社会培养更多的技能人才。

Studies on Techniques for Secondary Metabolites Culture in Vitro of Aloe

芦荟次生代谢物质离体培养技术的研究

If we do not attach importance to these phenomena , then there will be rural primary and secondary school culture , " emptiness ," scene .

如果我们不重视这些现象的话，农村中小学校园文化就会出现虚无化景象。

Twenty-first century has entered the information age , aspects of social life have become increasingly close ties with the information , which strengthen the secondary class culture of the modern information environment provided the preconditions .

二十一世纪已步入信息时代，社会生活的方方面面都与信息有着越来越紧密的联系，这为现代信息环境下加强中学班级文化建设提供了前提条件。

Strengthening the Secondary School Campus Culture by Promoting Folk Culture

弘扬民俗文化，加强中学校园文化建设

The secondary school basketball culture has its own distinctive character , and focused on basketball in the entertainment and education .

中学校园篮球文化具有独特的显著特征，其重点在娱乐和教育。

The standardized production was realized by fine management of secondary spawn and culture spawn for edible mushrooms .

对食用菌原种和栽培种生产进行细化管理，实现其规范操作、规范生产。

And ( 4 ) production of stock seed free of diseases . Fine Management of Edible Fungi Secondary Spawn and Culture Spawn Production

抗病原种的生产。食用菌原种和栽培种生产细化管理

Although the secondary school basketball culture is well positioned to , all secondary school basketball culture is not non-synchronization . , so the secondary school basketball culture is in a stage of exploration and to improve .

虽然中学校园篮球文化建设具备优越的条件，但由于各个中学的实际情况不同，导致校园篮球文化建设具有非同步性，因此，中学校园篮球文化建设正处于探索和提高阶段。

During the research of enhancing the production output of secondary metabolites , immobilization culture shows the advantages gradually .

在提高次级代谢物产量的研究中，固定化培养逐步显示其优势。

However , for secondary vocational schools , culture teaching is a new concept , and it has never got its due attention .

但是，对于中等职业学校而言，文化教学还是一个崭新的概念，它在中等职业教育中还没有得到足够的重视。

The fourth festival from four aspects analyzes Hunan TongDao Dong Autonomous County primary and secondary school teachers ' culture .

第四节从四个方面对湖南通道侗族自治县中小学教师文化进行了具体的考察和分析。

Objective To compare the survival situation between the direct isolation and after cell transplantation of IPE of secondary generation in vitro culture .

目的比较直接分离和体外培养的继二代有色素家兔虹膜色素上皮（IPE）细胞移植于无色素家兔视网膜下间隙后的存活状况。

Methods The IPE were isolated with enzyme-anatomy-enzyme method and cell suspension was prepared with IPE after secondary generation in vitro culture .

方法分别选用经酶-显微解剖-酶分离法直接分离的有色素家兔虹膜色素上皮细胞，和经过体外培养至继二代的有色素家兔虹膜色素上皮细胞，制成相同密度的IPE细胞悬液。