Pronase

The ventricular myocytes were prepared from guinea pig by the enzymatic dissociation with pronase E ( 0.5 ? mg / ml ) solutions .

用链霉蛋白酶E（0.5mgmL）灌流消化、分离得到豚鼠心室肌细胞。

Pronase , Rabbit anti-Streptomyces griseus , Biotin .

链霉蛋白酶，兔抗链酶菌，生物素。

Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation .

方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。

However combination treatment by collagenase and pronase , the cell number , the proportion of epithelia cell or the cell viability are high .

而胶原蛋白酶和链霉蛋白酶联合使用时，得到的细胞总数、上皮细胞比率及细胞活率都较高。

It was found to be tolerant to Pronase E and trypsin and partially sensitive to proteinase K ;

抗菌蛋白对链霉蛋白酶E和胰蛋白酶不敏感，对蛋白酶K部分敏感；

By using twice pronase zymolysis , complex action and UF , the HA intermidiary was purified .

采用链霉蛋白酶二次酶解、络合沉淀和超滤结合的方法，对HA中间品进行纯化处理，制得了高纯度的HA精品。

Crude polysaccharide was obtained from Agaricus blazei Murill fruiting body by hot water extraction , ethanol precipitation and pronase treatment .

从巴西蘑菇子实体中提取的水溶性粗多糖经过脱蛋白、脱色等步骤获得半纯品多糖。

ASMCs were isolated with pronase E and mesenteric arteries A2 and A3 from sham and shock rats also isolated .

急性分离大鼠肠系膜A2、A3动脉，链霉蛋白酶法急性分离血管平滑肌细胞；

After digestion of core protein by pronase , they released correspondingly glycosaminoglycan chains of 30 , 2.2 and 150 kDa .

用链霉蛋白酶水解去角蛋白后，它们各自释放的相应的氨基葡聚糖链为30,2.2150kDa。

Results Neurons isolated by pronase E were suitable for single record as examined by morphological observation and patch-clamp method , even though the death rates of neurons were no difference ;

结果经蛋白酶E或胰蛋白酶分离法得到的细胞在进行单细胞记录时虽然细胞的死亡率没有区别，但形态学、膜片钳等实验均表明蛋白酶E分离法分离的神经元活性更好；

The antagonism of iRNA is sensitive to RNase but not to DNase or pronase .

iRNA的这两种对抗效应均对RNase敏感而耐受DNase和Pronase。

The optimum Pronase E zymolysis condition : temperature is 40C , time is 12h , PH is 8.0 , the amount of Enzyme is 0.25 % .

链霉蛋白酶最佳酶解的条件是：温度为40℃，时间为12h，PH为8，酶量为0.25%；

Determining the lymphocytes digested by the combination of collagenase and pronase with comet assay , the result suggest that the DNA of the cells are not damaged .

用彗星电泳对胶原蛋白酶和链霉蛋白酶联合作用下的淋巴细胞进行了分析，结果表明，该条件不会对细胞的DNA造成损伤。

The antigenicity remained after treatment with RNase , DNase or pronase . The antigenicity , however , was lost when the preparation was treated with salivary amylase .

制剂经RNase、DNase或pronase处理后与抗血清仍有沉淀反应，但经唾液淀粉酶处理后沉淀线消失。

Single rat tail arterial smooth muscle cells were prepared by enzymatic digestion with pronase E ( 1 ? mg / ml ) and collagenase ⅰ( 1 ? mg / ml ) .

用链霉蛋白酶E（1mgmL）、胶原酶Ⅰ（1mgmL）消化分离大鼠尾动脉平滑肌细胞；

Methods : RHCPNwere obtained by pronase E treatment and then by trituration with pipette from 7 to 21 day-old rats . PCT was used to study their electrophysiological properties .

方法：用7～21d大鼠，以链霉蛋白酶E酶解加吸管吹打法制备RHCPN，用全细胞PCT测定其电生理学特性。

B.by a microblade after removal of the zonae pellucide by digestion with pronase ;

B、酶消化透明带后分割；

The sperm-oocyte binding was markedly inhibited by the glycopeptide from BSL degradated by pronase E , suggesting that the glycopeptide of BSL may also take part in sperm-oocyte binding .

经链霉蛋白酶E酶解获得的糖肽显著抑制精卵结合，提示BSL糖肽亦参与精卵结合。

Method : Non-parenchymal cells were fractionated by sequential collagenase and pronase digestion of liver . HSCs and KCs were separated by 18 % ( W / V ) Nycodenz .

方法：应用胶原酶和蛋白酶对大鼠肝脏进行原位灌流、消化，获得大鼠肝脏非实质细胞，经18%（W/V）的Nycodenz密度梯度离心，一步分离肝星形细胞及枯否细胞；

The glandular stomach was filled up with the enzyme solution ( 1.0 % pronase E ) and digested for 30-45 min in 37 ℃ . The GMC were collected by centrifuge , smeared and stained with Giemsa .

取腺胃，以1%链霉蛋白酶E于37℃消化30～45min，离心消化液收集胃粘膜细胞，制片，Giemsa染色。

Incubating the gastric mucosa in digestion solvent and shaking in 37 ℃ . Treated by collagenase , pronase , trypsin , EDTA-Na_2 separately , and combination of EDTA-Na_2 and trypsin , the cell number released from every sample is low .

将胃粘膜组织置于不同分离液中，37℃振荡50min，结果显示：胶原蛋白酶、链霉蛋白酶、胰酶、EDTA-Na2单独使用及胰酶与EDTA-Na2联合使用，样本得到的细胞都较少；

Methods : 3H labeled N linked sugar chains were extracted from the surface of HL 60 cells using pronase digestion after induced differentiation of the cells , and the structure of N sugar chains were analyzed with serial lectin chromatography , combining with exoglycosidase treatment .

方法：通过蛋白酶消化提取细胞表面3H标记N糖链，结合外切糖苷酶处理，经序列凝集素亲和层析分析HL60细胞诱导分化后细胞表面N糖链结构。

The ABS was sensitive to the action of proteolytic enzymes including trypsin , pronase E , and proteinase B.P ; but not sensitive to RNase and DNase . It was not inactivated by heat at 75-80 ℃ for 1 h.

对蛋白分解酶敏感，包括胰蛋白酶、蛋白酶E、蛋白酶B.P，抗DNase及RNase；

Methods : FSC were isolated with method of density gradient centrifugation after digest in pronase E and collagenase , and then observed the effect of TFP on proliferation and collagen synthesis of FSC , by means of MTT , the measuring incorporation of 3H-proline .

方法：采用链酶蛋白酶、胶原酶及密度梯度离心，分离大鼠肝脏贮脂细胞，用MTT方法及~3H-脯氨酸掺入方法，观察了三氟拉嗪对贮脂细胞增殖的调控。

Objective The present study was to elucidate whether there was a local renin-angiotensin-aldosterone system ( RAAS ) in rat hepatic stellate cells ( HSC ) and the immortalized rat HSC line & HSC-T6 . Methods HSC was isolated by perfusion of collagenase and pronase to liver .

目的明确肝星形细胞和永生肝星形细胞株HSCT6是否存在局部肾素血管紧张素醛固酮系统。

Cryostat and paraffin section are stained with ABC technique in comparison with the BT staining method . lgD expression in lymphocytes on paraffin section of human tonsils is detected with BT method combined with the pretreatments of pronase E digestion , microwave radiation and some detergents .

本研究以冰冻切片及石蜡切片中ABC法为对照，结合微波和蛋白酶消化预处理，采用BT法检测了人扁桃体石蜡切片标本中淋巴细胞IgD的表达。