Primase

The establishment of a screening method of DNA primase inhibitors

DNA引物酶抑制剂筛选方法的建立及应用

Extraction and primer synthesis activity of DNA primase

DNA引物酶的提取分离与引物合成活性的研究

DNA primase activity was assayed with E coli . DNA polymerase ⅰ Klenow fragment extended primers . The specific activity of the enzyme was 8 753 U / mg protein .

用大肠杆菌大片段DNA多聚酶Ⅰ延长引物法检测DNA引物酶比活性为8753U／mg，酶总获得为22.1%。

DNA polymerase α - DNA primase complex from K562 cells , a human chronic myelogenous leukemia cell strain , was purified by sequential chromatography of phosphocellulose , DEAE-cellulose , single-strand DNA cellulose and AMP-sepharose columns .

用磷酸纤维素、DEAE纤维素、单链DNA纤维素和AMP-Sepharcse的顺序柱层析法从人慢性骨髓细胞性白血病K562细胞中纯化了DNA聚酶α与DNA引物酶的复合物。