Peptide Mapping

A Preliminary investigation in the alteration of peptide mapping and its promotion to the growth during Sufu fermentation

腐乳发酵过程中肽谱变化及其促生长性能初探

Objective : Establish the method for peptide mapping of rhG-CSF .

目的：建立检测重组人粒细胞集落刺激因子（rhG-CSF）肽谱的方法。

Peptide Mapping and Primary Structure Analysis of hEGF by Mass Spectrometry

人表皮生长因子肽谱及一级结构的质谱法分析

Rule Effecting Peptide Mapping from Dynamic Factor in Reverse Phase Liquid Chromatography

反相液相色谱中动力学因素对肽谱影响规律研究

Peptide Mapping of Recombinant Human Epidermal Growth Factor by RP-HPLC

应用RP-HPLC分析基因工程人表皮生长因子肽谱

METHODS The peptide mapping was analyzed by trypsin digestion and RP-HPLC analysis .

方法采用胰蛋白酶裂解-反相高效液相色谱法测定肽图；

Conclusions : The method was suitable for the peptide mapping of rhG-CSF .

结论：本法适用于基因工程药物的肽谱分析。

Study on peptide mapping analysis of recombinant medicine rhG-CSF with microcolumn HPLC

微柱高效液相色谱分析基因工程药物rhG-CSF肽谱的应用研究

The peptide mapping and amino acids of Cerebrolysin were studied by using HPLC .

本文采用高效液相色谱法分析研究了进口药品脑活素注射液中的氨基酸含量及其低分子肽的肽图。

Peptide Mapping Analysis of Recombinant Human Neu Epitope Peptide 12 by Pepsin Digestion

重组人纽表位肽12的胃蛋白酶切肽图分析方法的研究

Analysis of Trypsinase Peptide Mapping of Trichosanthin by High-Performance Capillary Zone Electrophoresis

天花粉蛋白胰酶肽图谱的毛细管区带电泳法分析

Identification tests were done by peptide mapping , western blot and N - terminal amino acid sequence .

用肽图、免疫印迹及N-端氨基酸序列测定分析、鉴定产品特性；

Peptide mapping analysis of recombinant human interleukin-11 with HPLC-ESI-Q-TOF / MS spectrometry

液质联用分析重组人白细胞介素-11的肽图

Trypsin Immobilized in Sol-gel for High-throughput Protein Peptide Mapping

溶胶凝胶膜包埋的胰蛋白酶用于高通量蛋白质肽谱分析

Purity and peptide mapping determination of recombinant human calcineurin B subunit by HPLC and MALDI-TOF-MS

HPLC和MALDI-TOF-MS法分析重组人钙调磷酸酶B亚基的纯度和肽图谱

The Determining of the Amino Acid Contents and the Peptide Mapping of Injection of Cerebroprotein Hydrolysate

脑蛋白水解物注射液氨基酸含量及肽图测定

Analysis of Trypsinase Peptide Mapping of Trichosanthin by Reverse - phase High Performance Liquid Chromatogram and MALDI-TOF-MS

高效液相色谱和基质辅助激光解析电离飞行时间质谱法分析天花粉蛋白胰酶肽图谱

The acidic polypeptide of glutelin could be clearly separated into two groups by peptide mapping and N_terminal amino acid sequence analysis .

肽图谱与N端氨基酸序列分析可清晰将谷蛋白酸性多肽分为两组。

The peptide mapping for a protein by combination of the immobilized enzyme membrane microreactor and MALDI-TOF-MShas been developed .

固定化酶反应器被应用于蛋白质酶解的肽谱实验。

Comparative Study on Tryptic Peptide Mapping of Globin Beta Chains and the Genotype from Nineteen Inbred Strains of Mice

19个近交品系小鼠珠蛋白β肽谱的建立及遗传型的比较

Conclusion : The method is suitable for the peptide mapping of TCS , and it also provides a selective analysis method for TCS protein drug by PEG modification .

结论：本法适应于天花粉蛋白药物的肽谱分析，同时也为分析PEG修饰天花粉蛋白药物肽图谱提供了可以借鉴定手段。

Determination of peptide mapping with and without reducing agent followed by MALDI-TOF-MS indicated that the cysteine 70 is linked with the cysteine 158 by disulfide bond .

通过比较存在或缺少还原剂条件下的肽谱差异，并依据MALDI-TOF-MS的分析结果，证实EDGP的半胱氨酸70与半胱氨酸158之间形成了二硫键。

Methods : Various analytical techniques including gene sequencing , amino acid composition , analysis of N terminal amino acid analysis , peptide mapping , IEF , molecular weight and UV scanning were used .

方法：用基因测序、氨基酸组成分析、N末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L天冬酰胺酶及其天然品进行了全面比较。

The purity of recombinant Helicobacter pylori B subunit of urease vaccine trial production was analysed , the peptide mapping was made , it primary structure and it stabilization of production technics was approve .

分析重组幽门螺杆菌尿素酶B亚单位疫苗（rUreB）中试产品的纯度，制备肽指纹图，证明rUreB三批中试产品在一级结构上的一致性和生产工艺稳定性。

Objective : To establish the method of tryptic peptide mapping for recombinant human parathyroid hor - mone [ rh-PTH ( 1-34 ) ] and then determine its peptide map .

目的：建立可用于质量控制的rh-PTH（1-34）的标准胰酶肽图分析方法，并确定其理论肽图。

AIM To establish a standard method of peptide mapping analysis for quality control of rhIL 11 . METHODS To find out the best tryptic digestion and chromatogram conditions by using Alliance HPLC system and automatic temperature controlled injector .

目的建立标准肽图分析法，用于rhIL11的质量控制。

The glycosylated site was determined by comparison of peptide mapping before and after glycanase treatment . Tandem MS ( MS / MS ) was performed to analyze the structure of N linked glycan and deglycosylated peptide .

通过比较糖苷酶处理酶解肽段前后的肽谱，确定糖基化位点，并通过串联质谱（MS／MS）解析了Asn连接的糖型结构及去糖后肽段的氨基酸序列。

Because the conventional in-solution digestion of proteins is time-consuming and the autolysis of protease may generate interfering fragments , it is important to develop novel methods to achieve a highly efficient proteolysis for MS-based peptide mapping .

因为在溶液中进行的传统酶解方法非常耗时，且蛋白酶的自溶作用会产生干扰碎片，所以开发新的方法以达到基于质谱的肽谱法所需的蛋白高效酶解是很重要的。

A sensitive and simplified method of ~ ( 125 ) I-labeled peptide mapping was used to estimate the rates of the haemagglutinin ( HA ) mutation of 5 strains of influenza Al viruses and 5 strains of influenza A3 viruses .

将一种灵敏而简便的~（125）Ⅰ标记肽图分析方法用于估计近年来流行的5株甲1型和S株甲3型流感病毒血凝素（HA）的变异率。

Therefore , the possibility to detect the thiophosphorylated and fluorophore labeled peptide during high performance liquid chromatography peptide mapping was primarily shown .

初步显示高效液相色谱蛋白酶解肽谱分析中，荧光检测硫代磷酸化荧光标记肽的可能性。