After the blastocysts were hatched naturally from the zone pellucida , the inner cell mass ( ICM ) was dislodged from blastocysts using the sealed end of a finely drawn Pasteur pipette and co cultured with the feeder cells .
当囊胚由透明带自然孵出后,用细玻璃针剥离囊胚中的内细胞团(innercellmass,ICM)并与饲养细胞进行共培养。