PNPP

Studies on PNPP hydrolysis catalyzed by Schiff base cobalt (ⅱ) complexes

Schiff碱钴（Ⅱ）配合物催化PNPP水解研究

The activity of alkaline phosphatase ( ALP ) was measured with PNPP method .

PNPP法测定碱性磷酸酶（ALP）活性；

In addition , the complexes with tetrahedral structure would accelerate the hydrolysis of PNPP .

配合物的空间构型对反应速率有较大影响，具有四面体结构的配合物更有利于PNPP的水解。

A kinetic mathematical model of PNPP cleavage catalyzed by dinuclear copper ( II ) complex was proposed .

提出了含两个水分子的双核铜配合物催化PNPP水解的动力学数学模型。

Kinetic Investigation on the Hydrolysis of PNPP and PNPA by Two Long Alkyl Pyridine Metal Complexes

两种长链吡啶类金属配合物催化PNPP和PNPA水解的动力学研究

The alkaline phosphatase ( ALP ) activity of MSCs was detected by PNPP assay .

PNPP偶氮法检测MSCs碱性磷酸酶（ALP）的活性；

Synthesis of multi-functional macrocyclic polyamines La (ⅲ) complex and its catalytic activity for hydrolysis of PNPP

多功能臂大环多胺La（Ⅲ）配合物的合成及其对PNPP催化水解研究

Thekinetics and the mechanism on PNPP hydrolysis catalyzed by these complexes were investigated .

探讨了聚醚桥连二异羟肟酸过渡金属配合物催化PNPP水解的动力学和机理；

The purification multiple was 77.24 , and the specific activity was 16.22 unit / mg . Pr with pNPP as its substrate .

提纯倍数77.24，酶液比活力为16.22U／mg蛋白（对硝基苯磷酸二钠作底物）。

MTT assay and PNPP assay were used to observe the effects of different concentrations of rosiglitazone on proliferation and differentiation of the cell .

应用MTT法和PNPP法检测不同浓度罗格列酮刺激对大鼠成骨细胞增殖和ALP活性的影响。

Effects of β - Cyclodextrin on Catalytic Hydrolysis of PNPP and PNPA in CTAB Micellar Solution

β-环糊精对CTAB胶束催化羧酸酯水解的影响

The results showed that the MnL 1_2Cl can effectively catalyze PNPP hydrolysis under a neutral or basic condition ;

结果表明，在中性、碱性和室温（25℃）条件下，MnL12Cl能够有效地催化水解PNPP。

Phosphorus-starved cells can utilize G-6-P and pNPP to support some growth in the absence of inorganic phosphorus .

磷饥饿细胞在无机磷不存在的条件下，能利用有机磷G-6-P和pNPP获得部分生长。

Study on Aza Crown Ether Substituted Unsymmetry Bis-schiff base Complexes as Mimic Hydrolytic Enzyme Catalyze PNPP Hydrolysis

氮杂冠醚取代非对称双Schiff碱配合物模拟水解酶催化PNPP水解研究

The rate of the PNPP catalytic hydrolysis is increased following increasing pH value of the buffer solution and affected by the polarization action of metal ion of complex .

在缓冲溶液中随着溶液pH的增大，PNPP催化水解速率提高；配合物中金属离子极化作用影响PNPP催化水解速率。

MTT , PNPP and ARS were used to observe the proliferation , activity of ALP and the number of mineral node of cultured osteoblasts in vitro .

应用MTT法，对硝基苯磷酸盐法（PNPP）及茜素红染色方法观察补肾活血方对体外培养成骨细胞的增殖、碱性磷酸酶（ALP）活性及矿化结节形成的影响。

Then the catalytic activity of the com - plex on hydrolysis of p-nitrophenyl picolinate ( PNPP ) in buffer solution was studied as well .

最后研究了配合物对对硝基苯吡啶甲酸酯（PNPP）催化水解活性。

Methods : MTT , PNPP and BGP content radioimmunoassay were used to observe the proliferation , the activity of ALP and the BGP content of osteoblasts cultured in vitro .

方法：应用MTT，对硝基苯磷酸盐法及骨钙素含量放免测定法，观察丹仙康骨胶囊对成骨细胞增殖与分化作用的影响。

The results indicate that 1 catalyzed hydrolysis of PNPP and PNPA is acid base catalytic process and the active species is metal bound hydroxide ion , i.e. , CoL OH - .

结果表明：配合物1对PNPP及PNPA的催化作用具有酸碱催化的特征，催化活性物种为与金属离子结合的氢氧根离子CoL-OH-；

The Cu ( 2-AP ) 2 ( OAc ) 2 complex had no catalytic activity for the hydrolysis of PNPP and PNPA .

Cu（2AP）2（OAc）2对PNPP和PNPA均无催化活性。

By using PNPP as substrate , the subcellular distribution of calcineurin ( CaN ) activity was assayed in the supercentrifuged compartments in developing and adult rat cerebral cortex .

以PNPP为底物测定了超离心制备的大鼠出生后早期和成年大脑皮层亚细胞各组分中钙调神经磷酸酶的活力。

Two aza crown ether substituted unsymmetry bis-Schiff base transition-mental complexes have been used as mimic hydrolytic enzyme model catalyzing carboxylic acid esters ( PNPP ) hydrolysis .

将4种非对称双Schiff过渡金属配合物作为仿水解酶模型催化羧酸酯（PNPP）水解。

Cell adhesion was evaluated by counting cell numbers at 0.5h , 2h and 4h . The MTT assay and the pNPP phosphatase assay were used for cell proliferation and differentiation measurement .

于0.5.2和4小时进行细胞计数，计算细胞吸附率。分别利用MTT法，pNPP磷酸酶法评价细胞的增殖和分化。

[ METHOD ] The osteoblasts cultured with icariin of different concentration , MTT and PNPP methods were used to assay the proliferation and ALP activity of osteoblasts after 24 , 48 h and 72 h.

【方法】将不同浓度的淫羊藿苷加入第二代成骨细胞中，分别于培养24、48、72h时，使用MTT法和PNPP法检测成骨细胞的增殖率和碱性磷酸酶（ALP）活性。