G418

Results After G418 selection , the cell clones were obtained successfully .

结果经G418筛选后可形成抗性细胞克隆；

Conclusion When G418 has a high concentration , the result of the selection is better .

结论G418浓度高时，选择效果较好；

Apply of G418 Resistance Selection in Yeast System

G418抗性筛选标记在酵母体系中的应用

Positive clones were obtained by G418 screening after 10-15 days .

G418筛选10-15d后有抗性克隆形成。

After selecting with G418 , stable transfected cell line was gotten .

经G418筛选后，得到稳定转染的阳性细胞。

Resistant clone was selected with G418 and expanded in vitro .

G418筛选抗药性克隆进行扩大培养。

The screening systems for sugarcane calli with G418 were established .

建立了甘蔗G418筛选体系。

Conclusions It is a highly effective method to select the transfected cells using both G418 and FCM .

结论：合并使用G418及流式细胞仪分选是一种高效的筛选方法。

RESULTS : The stable cell line selected by G418 was identified by RT-PCR and Western blotting .

结果：经过8周G418筛选及RT-PCR和WESTERNBLOTTING鉴定，获得稳定表达PRL-2的细胞亚系PRL-2-CL1。

G418 was used to screen stable transfected cell and one single clone was separated by dilution .

用G418筛选出稳定转染细胞并分离出单克隆细胞株。

Secondly , we compare antibiotics both Kanamycin and G418 as selective pressure .

第二、比较了卡那霉素和G418作为抗性植株选择剂，以及合适的抗生素选择压力。

Results The cell clones expressing the interest protein were obtained by using G418 selection and MTX induction .

结果经G418筛选和MTX加压扩增，获得高效表达目的蛋白的细胞株。

Northern blotting analysis was used to check TKc gene expression in G418 resistant glioma cells .

Northern杂交检测抗G418胶质瘤细胞中的TKc基因表达。

Results The highest purity of GFP positive cells was 72.0 % when selected by G418 only ;

结果：单用G418筛选时最高筛选效率为72.0%；

Mensuration of the Minimal Inhibitory Concentration of G418 for Sugarcane Callus

甘蔗愈伤组织G418最低抑制浓度的测定

After transfection , positive clones were screened with G418 and expanded by culture .

G418筛选阳性克隆并扩增培养传代。

G418 and Chloramphenicol Work as Screening Markers of Transgenic Dunaliella salina

G418和氯霉素作为转基因盐藻的抗生素筛选标记

Results : Totally 192 ES cell lines survived G418 selection and homologous recombinant colonies were obtained .

结果：经G418筛选培养得到192个阳性克隆，分子鉴定获得2个发生同源重组的胚胎干细胞克隆。

Significantly TKc gene expression in these resistant glioma cells in G418 was found with Northern blot .

Northern杂交显示抗G418细胞TKc基因明显表达；

The results showed that the positive clones screened by G418 were mouse fibroblast cells with high expression level of VEGF .

结果显示：经G418筛选后的阳性细胞克隆，为高表达VEGF的鼠成纤维细胞模型。

Conditions for establishing mouse embryonic fibroblast feeder layers Preparation of a kind of G418 resistance fibroblast feeder layers

小鼠胚胎成纤维细胞饲养层制备条件的研究

However , the production rate of t-PA declined sharply following cell passage when there was no G418 existing in culture .

但在细胞传代中无G418压力时，t-PA表达水平迅速下降。

The monoclonal resisting cell strain screened by G418 was confirmed with RT-PCR and Northern Blot .

进而通过RT-PCR和NorthernBlot鉴定G418筛选后的单克隆抗性细胞株。

Observation of Correlation between G418 Selection Pressure and Stably Transfected hGM CSF Clones Expression and Secretion Level

G418选择与稳转克隆表达分泌hGM-CSF的相关性考察

The multicopy recombinants were obtained through G418 concentration gradient screening and PCR .

经G418浓度梯度筛选及PCR鉴定得到高拷贝整合的重组酵母表达菌株。

By G418 screening and colonies counting , gene pool A was chosen for the second cycle transfection .

经G418筛选并计集落数，选择克隆数最多的基因池进入第二轮。如此反复进行。

Transfected cells were selected by G418 to become stably transfected cells by using the biotechnologies of cloning .

转染细胞经G418筛选及克隆化培养后获得稳定转染株。

PK-15 cells expressing target gene were selected with G418 and identified by RT-PCR and IFA .

将pDisplay-mVP1转染PK15细胞，经3次G418加压筛选，并用RT-PCR和IFA鉴定，证明获得表达目的基因的PK15/pDisplay-mVP1细胞。

Results The positive cell clone selected by G418 system was proved to be a bile duct carcinoma model with high expression of β G.

结果经G418筛选后的阳性细胞克隆，为高表达βG的胆管癌模型。

The positive clones were screened by G418 and the expressive level of bFGF gene in positive cells was detected .

G418筛选阳性克隆，检测阳性细胞bFGF基因的表达水平。