Freund incomplete adjuvant

Methods Spleen cells of Balb / c mouse immunized subcutaneously with cysticercus fluid ( mixture of Freund 's incomplete adjuvant and interferon ) were fused with myeloma cells SP2 / 0 , and the resulting hybridoma cells were selected and cloned .

方法运用自提的猪囊虫囊液与福氏不完全佐剂加干扰素混合免疫Balb/c小鼠，取小鼠脾淋巴细胞与SP2/0骨髓瘤细胞融合，反复筛选及克隆化；

Fourteen days later , the same antigen emulsified in in freund 's incomplete adjuvant were intraperitoneally . and three days after final immunization , Splenocytes from the immunized mice were fused with Sp2 / 0-Ag-14 myeloma cells . Keep it in the bloom of proliferation before cell fusion .

然后每隔14天后再用弗氏不完全佐剂与之混合，共免疫2次，末次加强免疫以100μg／只尾静脉注射，3d后取小鼠脾细胞与骨髓瘤细胞Sp2／0-Ag-14进行融合。

With the chicken-derived anti-AIV IgG as the immune , the Freund 's complete adjuvant ( FCA ) vaccine and the Freund 's incomplete adjuvant ( FIA ) vaccine were prepared to incubated female SPF BALB / c mice of 8 weeks .

用以上提取的鸡抗AIVIgG作为免疫原，制备完弗氏全佐剂和不完全弗氏佐剂疫苗免疫8周龄雌性SPFBALB/c小鼠。