DNA transfection

Cell density was associated with PEI / DNA transfection efficiency .

细胞密度影响转染效率；

Expression of hepatitis B virus antigens in SD rat after HBV DNA transfection

乙型肝炎病毒基因注入大鼠肝脏后的短暂抗原表达

DNA Transfection of Insect Cells Efficiently Mediated by Stearylamine Liposome

SA脂质体介导DNA转染昆虫细胞的研究

Accelerated Somatic Cell Senescence and Changes in p16 ~ ( INK4a ) Expression after Exogenous DNA Transfection

外源基因转染导致小鼠体细胞过快衰老及p16~（INK4a）表达水平变化

Objective To study total nutrient admixture ( TNA ) promoting plasmid DNA transfection mediated with liposomes to colorectal cancer cells .

目的探讨全营养液（TNA）促进脂质体介导质粒DNA转染结直肠癌细胞的作用。

The proliferative and apoptosis rates of C6 cells were determined respectively by MTT and flow cytometry . The synergistic effect of Cx43 DNA transfection and radiotherapy on C6 cells was observed .

分别以MTT法及流式细胞仪检测C6、C6-Non、C6-Cx43细胞放疗组和未放疗组的细胞增殖率及凋亡率，进而观察转染Cx43基因与放疗杀伤胶质瘤细胞是否有协同作用。

The retrovirus carriers recombined , witch contains gene of bcl-2 mRNA cleaving RZ , were transfected into human cholangiocarcinoma cells QBC_ ( 939 ) by Lipofectamine intervened DNA transfection .

然后，采用脂质体Lipofectamine介导的DNA转染法，将重组后含有切割bcl-2mRNA核酶基因的真核细胞表达载体pDOR-RZ导入人胆管癌细胞系QBC（939）细胞内；

An Easy Available Modified Method of Calcium Phosphate Co - precipitation With DNA for Transfection of Mammalian Cells

一种磷酸钙DNA共沉淀转化哺乳化物细胞较易成功的简易变通方法

The DNA liposome transfection technology and insect cell culture technology were employed to culture and amplify the recombinant Bac TR ⅱ baculovirus particles .

采用DNA脂质体转染技术和昆虫细胞培养技术培养和扩增BacTRⅡ重组病毒颗粒；

Experimental studies were carried out by DNA transient transfection of cultured cells oncis-acting elements in the promoter and first intron of human type ⅱ collagen gene .

利用对培养细胞的短暂转染分析，对人Ⅱ型胶原蛋白基因启动子区和第一内含子区的四个可能的转录调控功能区进行了实验研究。

The Pharmaceutical Research on Antibody-conjugated Long-circulating Cationic Liposomes Mediating DNA and siRNA Transfection

抗体靶向的长循环阳离子脂质体介导DNA和siRNA转染的制剂学研究

Method s : DNA recombination , gene transfection and RT-PCR .

方法：重组克隆，基因转染及RT－PCR。

Freezing of PEI / DNA complexes significantly decreased transfection efficiency .

冻存显著降低PEI/DNA转染复合物转染效率。

Study on A Novel Non-viral Vector for DNA Delivery and Its Transfection Activity in the Cultured Cell

一种新的非病毒基因转移载体及其体外细胞转染活性研究

Influences of plasmids factor , albumin , serum , cell density , operation methods and polyethylenimine / DNA preserve factors on transfection efficiency were investigated .

我们通过聚乙烯亚胺转染编码β-半乳糖苷酶的pSVβ质粒到COS-7和NIH3T3细胞中，测定质粒因素、血清、细胞密度、操作方式以及转染复合物的保存因素对聚乙烯亚胺转基因效率的影响。

In this thesis , stearyl protamine as a kind of novel non-viral gene vector was synthesized and investigated on the characters of its DNA complexes and the transfection efficiency .

本文研究了硬脂酰鱼精蛋白的合成，并将其作为非病毒基因载体，考察了与质粒DNA的复合性能，复合物的体外基因转染效果。

Methods Molecular biology technique was used to extract mice oocytes , single-cell nested polymerase chain reaction ( PCR ) was used to detect HBV DNA in oocytes after transfection with PBR322-HBV DNA .

方法：用分子生物学技术提取、分离小鼠卵细胞，进行质粒PBR322-HBVdna的脂质体转染，采用单细胞巢式PCR检测卵细胞中携带的HBVdna；

In the present study , the effect of DOC - 1R expression on DNA replication and centrosome duplication was investigated using plas-mid DNA transfection and dual immunofluorescent microscopy .

我们结合转染技术及间接免疫荧光技术，观察细胞内DOC-1R转染基因表达对于DNA复制以及中心体复制的影响。

This review expounds the combination way of liposome and DNA , effects of immunoadjuvant of liposome in DNA vaccine , and transfection of liposome - DNA complexes .

本文对脂质体这种新型疫苗佐剂兼载体与DNA的结合方式、在DNA疫苗中的免疫佐剂作用、脂质体DNA复合物的转染及影响因素等方面作了比较详细地阐述。