CO2 Incubator

( 3 ) Experimental instruments : CO2 incubator , inverted microscope , low-oxygen incubator , fluorescence microscope , electro microscope . 2 . Methods ( 1 ) Rat brain microvascular endothelial cells culture and identification .

主要仪器：二氧化碳培养箱、倒置显微镜、低氧培养箱、荧光显微镜、透射电镜。（二）实验方法1、大鼠脑微血管内皮细胞的分离鉴定。

Growth culture medium was added into the remainder and inoculated in CO2 incubator .

再加入生长培养基重悬细胞，置于CO2孵箱中进行接种。

In 37C CO2 incubator cultured 2 days .

在37℃、体积分数为5%的CO。培养箱中培养Zd。

Methods The high metastasis adenoid cystic carcinoma cell lines SAS were contained in DMEM medium containing 10 % fetal bovine serum , 37 ℃, 5 % CO2 incubator in the closed-culture .

方法于含10%胎牛血清的DMEM培养基、37℃、含5%CO2的细胞培养箱中密闭培养口腔鳞状细胞癌细胞系SAS。

The cells in the control group were added with DMEM / F12 nutrient fluid of EGF , bFGF and B27 to culture in 5 % CO2 incubator for one week at 37 ℃ .

对照组细胞中直接加含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液，37℃，体积分数为0.05的CO2培养箱培养1周。

Hippocampal neurons dissected from the one-day newly-born rats can be cultured well in DMEM medium supplemented with 10 % fetal calf serum and 10 % new-born bovine serum in a 37 ℃, 5 % CO2 incubator .

新生一天的大鼠海马神经细胞在含10%胎牛血清和10%小牛血清的DMEM培养液，5%CO2,37℃的环境中可以获得良好的生长。

All cells were cultured at 37 ℃ in 5 % CO2 cell incubator .

各组在37℃、体积分数为0.05的CO2细胞培养箱内常规培养。

CO2 constant-temperature incubator ( QUEUE , USA ) .

CO2恒温培养箱（QUEUE，USA）。

Microencapsulation of islets of Langerhans ⅱ . The insulin secretion of the microencapsulated rat 's islets during in vitro culture using CO2 and common incubator

微囊包膜胰岛移植研究Ⅱ微囊包膜大鼠胰岛在CO2及普通培养箱中培养时对比研究

At 37 ℃, 5 % CO2 conditions in the incubator , each of the 12-24h change 1 time depending on cell growth , in cell close to the shops flasks or training Board ( 4-5 days ) for generations .

在37℃、5%C02条件下的恒温培养箱中进行培养，每12-24h换液1次，视细胞生长情况，在细胞接近铺满培养瓶或培养板底时（4-5天后）进行传代。