CCAAT box
- 网络ccaat盒;CCAAT框
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W box , X box , Y box and TATA-like box were polymorphic , CCAAT box was conserved . Polymorphic sites also existed between the regulatory elements .
其中WBOX、Xbox、YBOX及类TATABox是多态的,CCAATbox是保守的,在这些调控元件之间也存在多态座位。
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HAP , a kind of important transcription factor , occurs in yeasts , mammals and plants . It can bind to the CCAAT box and regulate the expression of target genes .
HAP是与CCAAT框结合的非常重要的一类转录因子,控制基因的表达,并广泛分布于酵母、哺乳动物、植物细胞中。
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Regulatory elements W box , X box , Y box , CCAAT box and TATA-like box which were associated with the expression of structural gene were found in all the URR sequences .
这些序列中均存在与基因表达调控有关的WBOX、Xbox、YBOX、CCAATbox以及类TATABox调控元件,且遵循严格的空间顺序。
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To functionally determine the importance of these elements , site-directed mutagenesis was performed and three mutants were constructed respectively , containing a single mutated CCAAT box , a single mutated TCCC box and their double mutant .
为了探讨这些转录元件的生物学功能,本研究利用定点突变技术构建了3个突变体,分别为CCAATbox单突变、TCCCbox单突变和二者的双突变。