Blastomere

Establishment of a FISH Method for HBV Detection in Human Blastomere

卵裂球细胞乙型肝炎病毒FISH检测法的建立

The amplification success rate of single blastomere was 75.4 % .

单卵裂球的扩增成功率为75.4%。

Conclusion : LN existed in the blastomere during early mouse embryo development ;

结论：早期小鼠胚胎的卵裂球中含有LN；

Impact of blastomere survival on implantation rate of frozen-thawed embryo transfer

卵裂球的存活状况对冻融胚胎种植率的影响

Research on the Pre-treatment Technique for Single Blastomere before Fluorescence in Situ Hybridization

单个卵裂球荧光原位杂交标本前处理的研究

Conclusion The oviduct is the site of sperm-egg interaction and blastomere embryo development .

结论输卵管是精子与卵子结合的地方，也是胚胎早期发育的场所。

Effect of ROS on the numbers of blastomere and mitotic index in mouse embryos

活性氧（ROS）对小鼠早期胚胎胚细胞分裂的影响

CONCLUSION : Blastomere survival status is a most important factor influencing the implantation rate of frozen-thawed embryo transfer .

结论：卵裂球的存活状况是影响冻融胚胎种植率的重要因素。

Hence , potentially viable embryos will be discarded upon screening a single blastomere .

因此，有可能对单个卵裂球的基因筛选导致一个有活力的胚胎被丢弃。

Impact of blastomere number and fragment on the blastomere survival of human cryopreserved embryos

卵裂球数量及胚胎碎片对人冻胚卵裂球存活状况的影响

Conclusion : Single blastomere PCR is stable and reliable , and it can be used for preimplantation genetic diagnosis .

目的建立单卵裂球PCR技术，为开展单基因病的着床前遗传学诊断奠定基础。

Cell embryos blastomere of Xiang pig were used as nuclear donor and Shanghai White pig used as nuclear recipient .

选用香猪2～16细胞期卵裂球作核供体，上海白猪卵母细胞作核受体。

The ES cell lines establishment efficiency from a single blastomere of embryo is inversely proportional to the embryo development stages .

单卵裂球建立ES细胞的成功率与胚胎发育阶段负相关。

In morula , positive LN immunoreactive substance appeared simultaneously in both blastomere and extracellular matrix .

到桑椹胚期，卵裂球内及间质中同时出现LN阳性反应物质。

The amplification efficiencies of multiplex nested PCR of single blastomere was ( 94.1 % ) .

单卵裂球的扩增效率为94.1%。

The average number of blastomere and score of embryo in D3 were much higher than D2 , P < 0.01 ;

D3妊娠组所移植胚胎的平均卵裂球数及评分显著高于D2组、P<0.01；

Chromosomal diagnosis in each individual blastomere of 5-to 10-cell bovine embryos derived from in vitro fertilization

牛体外受精卵5～10细胞期单一卵裂球染色体诊断

Early embryonic cell has the developmental totipotency . Every embryonic blastomere cell can develop to an intact individual separately in a proper condition .

早期胚胎卵裂球细胞具有发育全能性，每个胚胎卵裂球细胞在适宜的条件下都能单独发育为一个完整的个体。

There was no certain regulation in the distributing of the progeny of 2-cell embryo blastomere in blastocyst .

实验结果如下：1.2-细胞胚胎两个卵裂球的后代在囊胚中分布并没有一定的规律。

The fusion rate of the oocyte blastomere pairs which were not fused for the first time decreased remarkably when they were pulsed for the second time .

一次电刺激后未发生融合的注卵裂球胚胎再次电刺激时，其融合率显著降低，由51.3%降至10.5%；

The fuse rate of rabbit 32-cell stage embryo blastomere and mouse enucleated M ⅱ oocytes were 66 % .

兔32-细胞期胚胎卵裂球与小鼠去核的MⅡ期卵母细胞融合率为66%。

Use HCL / Tween20to fix blastomere .

采用HCL/Tween20方法进行核固定。

Chromosomal normality and sex were diagnosed in each blastomere of bovine embryos derived from in vitro fertilization ( IVF ) .

利用单一卵裂球的染色体诊断牛体外受精5-～10-细胞期胚胎的正常性。

After ZGA , many kinds of glycoprotein antibodies can be produced by zygotic genes and transported to the surface of blastomere .

ZGA发生后，合子型基因编码的大量糖蛋白抗原可能分泌于胚胎细胞表面。

Results : A statistical difference was observed in the rate of embryo development , blastomere nuclei quantity and the type of fragmentation between the two groups ( P < 0.05 ) .

结果：Day3胚胎发育速度、卵裂球核数量和碎片类型在两组间差异有显著性（P<0.05）。

And the number of blastomere in FET embryo was significantly higher than fresh cycles ( most of which were D3 embryo ), P < 0.01 .

冷冻组的平均卵裂球数明显多于新鲜胚胎组（P<0.01）。

Objective : To evaluate the influence of the blastomere survival and resumption of mitosis on the clinical outcome in frozen-thawed embryo transfer ( FET ) cycles .

目的：分析冻融胚胎移植周期中胚胎卵裂球损伤程度与有丝分裂的恢复对于临床结局的影响。

A DC pulse of 1200v / cm and 80 μ s was applied to induce membrane fusion between a blastomere and an enucleated oocyte .

对组合卵施加一个1200伏/cm（V/cm）和80微秒（μs）的直流电脉冲，使卵裂球的细胞核导入去核卵母细胞之中。

At the CO 2 level of 15 % , 15 % embryos developed in the 4 cell stage with irregular blastomere and degenerated quickly in the group whose paraffin oil was treated with distilled water ;

CO2浓度15%时，覆盖经三蒸水处理的石蜡油组，有15%的胚胎发育到4-细胞阶段，但4-细胞胚胎的卵裂球不规则，很快退化；

Methods : 35 early human embryos ( 2-8 cell stage ) were isolated into single blastomere by the technic of embryo bisection , the blastomeres were cultured in vitro , and the development of the blastomeres was observed .

方法采用胚胎分割技术把35个人早期胚胎（2-8细胞期）分割成单个卵裂球，再行单个卵裂球的体外培养，观察卵裂球的生长发育情况。