293t

Effect of Caveolin-1 Overexpression on the Growth of 293T Cells and Its Mechanism

过表达小窝蛋白-1对293T细胞生长的影响及机制

Establishment of a High-Efficiency Calcium Phosphate Transfection Method for HEK 293T Cells

一种用磷酸钙法高效转染HEK293T细胞方法的建立

Construction of Eukaryotic Expression Vector of Human bFGF 、 KGF and Their Expression in 293T Cells

人碱性成纤维细胞因子、角朊细胞生长因子真核表达载体的构建及其在293T细胞中的表达

RT-PCR was performed to evaluate the transcription of gL in 293T cells .

RT-PCR方法检测gL基因在293T细胞中的转录。

Then , these recombinant plasmids were taken to infect 293T cells , respectively .

然后将每一个重组质粒分别转染293T细胞。

Our previous studies found that exogenous tau cDNA expressed in 293T cells could inhibit cell proliferation and differentiation .

我们以前的研究发现，外源性人taucDNA在细胞中过度表达对细胞的增殖与分化有抑制作用。

Overexpression and hyperphosphorylation of tau protein in stably transfected 293T cells

非神经源性细胞中tau蛋白的稳定表达及磷酸化

The plasmid was introduced into the 293T cells and we found their size and number of lipid droplets increased significantly .

通过转染293T细胞，发现其脂滴大小和数量明显增加。

PCR site-directed mutagenesis of avian influenza virus hemagglutinin gene in vitro and expression in 293T cell

禽流感病毒血凝素基因突变体的构建及其在293T细胞中的表达

Appling Comparative Proteomics Method to Study the Effect of p38 β on the Expression of Proteins in Human Thymus Tumor 293T Cells

采用比较蛋白质组学方法研究p38β激酶对293T细胞蛋白质表达的影响

Conclusion Human CD23 cDNA was amplified and the high-level expression of CD23 in 293T cells was achieved .

结论扩增CD23cDNA基因，经过转染HEK293T细胞，实现了CD23在HEK293T细胞的高效表达。

The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA .

用此SHIV原病毒DNA转染293T细胞，细胞中能够检测到嵌合病毒基因的转录与翻译；

Then Co-IP method in mammalian cells 293T verified the authenticity of the interaction of the interaction between the two .

然后用Co-IP方法在哺乳动物细胞293T内验证相互作用的真实性两者的相互作用。

By Western blotting , the expression of HA protein was detected in 293T cell lysate transfected by HA-VN .

蛋白质印迹法分析结果表明，HA-VN.tPA转染293T细胞后，细胞裂解液中检测出HA蛋白的表达。

Effect of 293T / BTLA cells on T cells proliferation and activation in vitro was been studied by methods of MTT and cytometry .

通过MTT法和流式细胞术探讨基因转染细胞在体外对T淋巴细胞增殖与活化的影响。

Bcl-2 fusion protein had been detected successfully in transfected 293T cell lines , and the size of the fusion protein was the same as expected .

在转染后的293T细胞成功地检测出bcl-2融合蛋白的表达，融合蛋白大小与预期完全一致。

Transiently transfected 293T cells over-expression and access to intracellular proteins and extracellular ( secreted or membrane ) proteins and convenient way .

瞬时转染293FT细胞是过表达蛋白并获得细胞内及细胞外（分泌的或膜）蛋白的便捷方式。

The eight recombinant plasmids were transfected into 293T / MDCK mixed cell monolayer . Obvious cellular pathological changes could be observed after 72 hours .

将8个重组质粒转染293T／MDCK混合细胞单层，72h后出现明显细胞病变。

Secondly , the fusion proteins expressed in 293T cells were observed by fluorescence microscopy . The result showed that expression of DOG fusion protein was induced in hypoxia .

其次，将上述融合蛋白表达载体转入293T人胚肾细胞，借助荧光显微镜，观察不同融合蛋白在正常氧和缺氧细胞中的表达，发现DOG融合蛋白的稳定存在依赖于缺氧环境。

Conclusion : Glycoprotein K8.1 , K8.1A and gL of KSHV were correctly expressed in 293T cells .

结论：KSHVK8.1、K8.1A和gL编码基因在293T细胞中获得正确表达。

Results The expression levels of reporter gene induced by rosiglitazone in 293T cells was the highest , up to 4.9 fold , and in dose dependent manner .

结果293T细胞中，荧光素酶的表达受马来酸罗格列酮的诱导倍数最高，可达49倍，并呈现一定的剂量依赖关系，Z′因子为0.72。

Methods VSV-G / MuLV was produced from transient transfection of 293T / 17 cell line with 3 plasmids system with the method of calcium phosphate precipitation ;

方法利用三质粒系统一过性转染293T/17细胞，通过磷酸钙共沉淀法产生VSV-G/MuLV；一过性转染后的病毒上清转导包装细胞系293/GPC以产生VSV-G/MuLV生产细胞系。

The ectopic expression of XBP-1S and XBP-1U in 293T cells were confirmed by Western blotting and immunocytochemistry with the antiserum .

利用制备的抗体分别用Western印迹和免疫细胞化学检测XBP1的2种剪切形式在哺乳动物细胞中的表达。

Construction of eukaryotic expression vectors of carboxyl terminus and amino terminus of kinase suppressor of Ras ( KSR ) and their expression in 293T cell line

小鼠Ras激酶抑制剂（KSR）基因氨基端和羧基端真核表达载体的构建及其在293T细胞中的表达

By means of gene optimization , we have constructed human bFGF and KGF high-efficient eukaryotic expression vector in this experiment and they are transfered into cultural 293T cell to express ;

本实验通过基因优化等手段构建人bFGF和KGF的高效真核表达载体，并转染293T细胞获得表达；

The aim of this study is to construct eukaryotic expression vectors of carboxyl terminus and amino terminus of KSR and to detect their expression in 293T cell line .

本研究的目的是构建小鼠KSR基因氨基端（NKSR）和羧基端（CKSR）的真核表达载体，并观察其在293T细胞中的表达。

RESULTS : The recombinant plasmid was effectively transfected in 293T cells and green fluorescence could be observed under fluorescence microscopy in MMH-D3 cells from the supernatant of lentivirus effectively infected .

结果：重组慢病毒质粒高效率转染293T细胞，收取慢病毒上清转染MMH-D3细胞，荧光显微镜下可见大量绿色荧光。

Human embryonic kideny 293T cells were co-transfected with the three plasmids by calcium phosphate DNA precipitation and the expression of GFP was examined under fluorescent microscope 12 hours after transfection .

载体构建成功后，用磷酸钙沉淀法将三质粒共转染来源于人胚肾细胞系的包装细胞&293T，12h后在荧光显微镜下观察绿色荧光的表达情况。

Conclusion This is the first time for us to obtain the transgenic 293T cells expressing stably rhIL-17F protein in China , which paves the way for future study on biological functions and mechanism of hIL-17F .

结论以逆转录病毒为载体感染的稳定表达rhIL-17F的293T转基因细胞株的首次获得，为进一步研究hIL-17F的生物学功能及其机制奠定了良好的基础。

An interesting band about 35 ku was visible in the result of Western blot , which was consistent with expected size of recombinate protein of K8.1 and K8.1A expressed in 293T .

westernblot结果显示，在约35ku位置有目的条带，与预期的重组K8.1和K8.1A蛋白大小一致。