龟板

方法体外分离培养间充质干细胞，以龟板水煎液对其进行体外诱导，免疫组化鉴定NSE、Nestin、GFAP等标志。

Methods MSCs were separated and cultured in vitro and were induced by the decoction of tortoise plastron ( TP ), a Chinese traditional medicine with roles of invigorating the kidneys . NSE , NESTIN , GFAP were detected by immunohistochemistry .

生地、丹皮、鳖甲、龟板和知母等滋补肝肾药的使用频率依次为71%、57%、50%、43%和43%

Root of rehmannia , root bark of peony , turtle shell , tortoise plastron and rhizome of wind weed are 71 % , 57 % , 50 % , 43 % and 43 % .

柱前衍生化RP-HPLC法分析龟板中氨基酸

Determination of amino acid in Chinemys reevesii by pre-column derivatization RP-HPLC

调控鼠骨髓间质干细胞增殖的中药龟板化学成分的GC-MS测定

Effects on proliferation of rat mesenchymal stem cell and chemical components from Plastrum Testudinis obtained by GC-MS

目的采用柱前衍生RP-HPLC法对龟板中13种氨基酸进行分析。

Objective To determine the content of 13 amino acids in Chinemys reevesii by pre-column derivatization RP-HPLC .

[目的]观察益肾中药龟板血清对大鼠骨髓间质干细胞（MesenchymalStemcells，MSC）体外增殖的影响。

[ Objective ] To observe the effect of serum containing Carapax et Plastrum Testudinis ( CPT ) on proliferation of rat mesenchymal stem cells ( MSC ) .

【结果】龟板可降低神经病评分和增强脑缺血后Nestin表达。

Results Plastrum Testudinis can decrease the neurologic dificit score and promote the expression of Nestin .

中药龟板对Parkinson病大鼠模型BMP4表达的影响

Effects of plastrum testudinis on the expression of bone morphogenetic protein-4 in rat model of Parkinson 's disease

采用胰蛋白酶和中性蛋白酶复合水解龟板胶，同时以水解度为考察指标，用正交实验考查了影响酶解的四个主要因素（pH值、酶水解温度、底物浓度，酶水解时间）。

Using compound of trypsin and neutral protease to hydrolysis the glue of tortoise plastron , and studying the influence of the four main factors ( pH value , enzyme temperature , enzyme hydrolysis time , substrate concentration ) on the enzyme hydrolysis .

推测，核受体VDR协同龟板提取物在骨髓间充质干细胞向成骨分化过程中起到的重要的作用，其可能与BMP通路密切相关。

It is reasoned that the nuclear receptors VDR synergy of plastrum testudinis extracts in MSCs during osteogenic differentiation must play an important role , which may be closely related to BMP pathway .

通过构建大鼠Id1启动子证实了补益中药龟板提取物及其活性成分对骨髓间充质干细胞（MSCs）的增殖和分化的影响，并对促使其增殖的分子机制进行了初步的探讨。

By constructing a rat Idl promoter confirmed Extracts from Plastrum Testudinis promote ( PTE ) and active ingredients effected bone marrow stromal cells ( MSCs ) proliferation and differentiation and whose molecular mechanisms are primarily discussed .

[结果]不同浓度的龟板血清均以浓度依赖方式促进MSC增殖活力和增加Brdu、PCNA阳性细胞数，与对照组比较具有显著性差异（P<0.05或P<0.01）。

[ Results ] CPT-contained serum at different concentrations could promote the proliferation of MSC and increase the number of Brdu positive and PCNA-positive cells in a dose-dependant manner , the difference being significant in comparison with the control group ( P < 0.05 or P < 0.01 ) .

比值图示法鉴别龟板及其混淆品

Identification of Plastrum Testudinis and Its Adulterant by Ratio Diagrammatic Method

上面的龟板和下面的龟板在四周连接。

The top shell and the bottom shell join at the sides .

在非洲的岩石区域内，有的旱乌龟的龟板十分灵活。

In rocky areas of Africa , some tortoises have flexible shells .

龟板进化之后，甲壳也开始进化，这给了海龟更多的保护。

After the plastron evolved , the carapace developed , giving turtles extra protection .

龟板水提物缓释微球的制备及其特性

Preparation of Sustained Release Microspheres of Water Extract of Plastrum Testudinis and Their Properties

某些海龟在生长过程中，不断给它们的龟板增加“生长轮”。

Some turtles add " growing rings " to their shells as they grow .

本文报道了用比值图示法鉴别龟板及其混淆品。

The article reports the identification of Plastrum Testudinis and its adulterant by ratio diagrammatic method .

本课题以核受体为突破口，探讨龟板提取物对骨髓间充质干细胞成骨分化过程中核受体的影响。

To investigate plastrum testudinis extracts could promote MSCs differentiate into osteoblast and the process of nuclear receptors .

滋阴药生地龟板对甲亢大鼠肾脏β-肾上腺素能受体的调整作用

Regulatory Effect of Rehmannia Glutinose Libosch and Chinemys reevesii ( Yin Tonics ) on β - Adrenergic Receptor of Hyperthyroid Rat Kidney

目的研究龟板的体外抗氧化活性，为抗衰老药物的筛选提供参考。

OBJECTIVE : To study the antioxidant activities of Plastrum Testudinis in vitro so as to provide references for the optimization of antiaging agents .

海龙胶和龟板胶有升白细胞作用；鹿角胶有抗疲劳作用；

The number of white blood cells can be elevated by tortoise plastron glue and pipefish glue , deerhorn glue possesses an anti-fatigue effect ;

陆地龟，或者叫旱乌龟，是动作缓慢的动物，所以，它们通常有特别坚硬结实的龟板保护它们，避免敌人的伤害。

Land turtles , or tortoises , are slow-moving animals , so they usually have especially strong , tight shells to protect them from enemies .

对龟板的传统炮制品和食用菌炮制品进行了氨基酸、含氮量、浸出物、灰分以及微量元素等项目的系统分析。

Amino-acids , nitrogen content , soaking extracts , ash contents , trace elements have been systematically determined for the traditional preparation and food-bacteria preparation of Chinemys reevesii .

有北沙参、玉竹、麦冬、天冬、冬虫夏草、黄精、灵芝、石斛、女贞子、百合、柏子仁、龟板等。

North Adenophora , Polygonatum odoratum , Ophiopogon , Asparagus , Cordyceps , Wong Ching , Ganoderma lucidum , Dendrobium , Ligustrum lucidum , lilies , such as turtle shell .

目的在干细胞具先天之精属性新理论指导下，探讨补肾中药龟板对间充质干细胞的体外诱导作用。

Objective To investigate the differentiation of mesenchymal stem cells ( MSC ) into nerve system cells according to our new viewpoint that stem cells have the same properties of congenital essence .

方法选择较低的适宜温度油提生龟板、地黄和当归，摒弃油提液350℃高温长时间熬炼的传统方法。

Methods : Choose lower befitting tem - perature as to extract active Chinese drug component from fresh tortoise plastron , rehmannia root and angelica by oil , and dismiss the extracting oil , using the traditional method of long-time cooking at 350 ℃ .