脲酶

稻田脲酶抑制剂对尿素N利用效率的影响

Impact of Urease Inhibitors on Utilization Efficiency of N-Urea in Rice Paddy

脲酶Km值简易测定方法的改进

Improvement for A Simplified Way to Assay Urease K_m

根际土壤中自生固氮菌和硝化细菌的数量和脲酶的酶活性显著相关，P细菌和磷酸酶活性显著相关，说明这些菌对酶活的产生贡献比较大。

This suggested , these bacterial contributed enzyme activity .

脲酶抑制剂并不影响瘤胃的pH值，表明它不是通过改变瘤胃液pH值来发挥其抑制作用的。

The inhibiting effect was not caused by changing the pH value .

气隙式脲酶pH电极的研究

Study on pH based air - gap urea electrode

旱地土壤N2O排放与土壤脲酶活性关系的研究

Study on N_2O emission and urease activity in dryland soil

方法：采用脲酶实验及PCR方法检测幽门螺杆菌。

Methods : Urease test and PCR were used .

Zn添加量对土壤脲酶活性的影响表现为先上升后下降的趋势。

Soil urease activity increased first and then decreased at Zn application .

PCR法扩增脲酶B基因在快速诊断幽门螺杆菌感染中的应用研究

Application of PCR for the rapid diagnosis of Helicobacter Pylori infection by amplification of urease B gene

基于等离子体修饰的PVC膜脲酶传感器

New Urease Biosensor Based on the Plasma-Modified pH-Sensitive PVC Membrane

通过X射线微区分析可知脲酶在膜上的分布是均匀的；

The localization of activity of urease immobilized on porous chitosan membrane was studied by X-ray microanalysis .

脲酶结合物ELISA测定法在日本血吸虫病诊断中应用的初步报告

Urease-antibody conjugate ELISA and its application in the diagnosis of schistosomiasis Japonica-A preliminary report

土壤酶活性均在E（肥土比1：200）施肥量时发生明显变化，脲酶、中性磷酸酶和蔗糖酶活性急剧下降。

Activity of urease , neutral phosphatase and invertase significantly decreased in the E ( 1:200 ) treatment .

PVA固定化脲酶电极的制作及电极特性的研究

Making and Characteristics of a PVA Immobilized Urease Electrode

控释氮肥氨挥发量与不同氮肥引起的土壤脲酶活性、pH值、土壤中氮溶出速率密切相关。

The results also indicated that total ammonia volatilization correlated significantly with soil urease activity , pH value and N leaching rate .

同时对固定化和游离脲酶的km、最佳反应温度、最佳反应pH、稳定性等进行了研究。

We also have research the km and the reaction condition and the stability of free and immobilized urease .

堆肥可显著提高Cd污染土壤中脲酶、蔗糖酶、磷酸酶及蛋白酶的活性，改善土壤生化性质。

Compost application increased the activity of sucrase , urease , phosphatase and proteinase , improved the soil biochemistry property .

Se4+处理的大豆N、P含量和土壤脲酶和磷酸酶活性均无显著相关关系。

There were no significant relationships between the N and P contents of soybean and the activities of test soil enzymes when Se 4 + was applied .

土壤脲酶活性随Cd添加量的增加总体呈下降趋势，说明Cd抑制了土壤脲酶的活性。

Soil urease activity had the overall declining trend with increasing Cd concentration , indicating that Cd inhibited soil urease activity .

结果表明，在脲酶（UA）完全失活条件下，胰蛋白酶抑制素（TI）仍残留一定活性。

The results indicated that the TI still remained certain activity when UA fully deactivated .

土壤中有效Zn、Pb、Cd与土壤微生物量碳、磷酸酶活性和脲酶活性呈负相关趋势，并没有达到显著相关关系。

Negative correlated was founded between the available heavy metals contents and soil microbial biomass , phosphatase activity and urease activity .

施N干扰可使糙皮桦林地土壤脲酶、过氧化氢酶和蔗糖酶活性较对照有不同程度的提高，而施C对土壤过氧化氢酶活性影响不明显；

N disturbances can make B.utilis soil urease , catalase , invertase enzymes activities improve in various degrees , C disturbances did not influence on the catalase enzyme activity obviously ;

脲酶与pH值和细菌含量呈显著负相关；

The negative correlation of urease activity with soil pH and bacteria amount , and acid phosphatase activity with the content of available iron were also found prominently .

重牧后的恢复草地表层土壤较其它处理有较高的土壤容重和pH值以及较低的过氧化氢酶、脲酶和碱性磷酸酶活性；

Soils in post-heavy-grazing restoration plot exhibit higher bulk density and pH values , and lower Catalase , Urease , and alkaline Phosphatase activities compared to other plots .

结果表明，PVA凝胶固定化脲酶具有较高的活力收率（79%）。

Comparative studies show that the immobilized urease on PVA gel has a higher activity yield ( 79 % ) .

具体研究内容如下：1.发展了一种基于等离子体修饰的PVC膜脲酶生物传感器。

The detail contents list as follows : 1 . A new urease biosensor based on the plasma modified PVC membrane has been developed .

kg-1.脲酶对Hg污染比转化酶敏感；有机质对土壤酶活性有一定的保护作用。

Urease was more sensitive than invertase to Hg contamination , while organic matter had a protective effect on soil enzymes .

试验表明，施用壳聚糖包膜尿素能降低土壤的脲酶活性，提高过氧化氢酶活性；同时降低了土壤的EC值，但对土壤的pH影响不大。

During late period , also debasing urease activity in soil , increasing catalase activity , lowering the value of EC , little effect on pH were found .

地膜覆盖能使土壤N2O排放量和耕层不同层次中的脲酶活性升高；

The plastic film mulching would increase the N 2O emission and urease activity in different soil layers .

阐述了脲酶抑制剂氢醌对土壤中的尿素NH3挥发有抑制作用，延缓尿素水解速率。

Urea inhibitor HQ can retard urea hydrolysis rate and loss of ammonium to air by volatilization .