细胞原

SD大鼠胸主动脉平滑肌细胞原代培养及鉴定

Primary culture and identification of SD rat aortic smooth muscle cells

肺癌细胞原代培养MTT法药敏试验研究

Study of drug sensitivity of lung cancer cells with MTT

Wistar大鼠晶体上皮细胞原代培养及鉴定

Primary culture and identification of wistar rat lens epithelial cells

神经干细胞原代培养及GABA能神经元的诱导分化

Primary culture of neural stem cells and induction and differentiation of GABA neurons

材料与方法：1、应用分步消化法进行Wistar大鼠乳鼠心肌细胞原代培养，采用特异性免疫细胞化学染色方法鉴定所培养的心肌细胞。

Methods : 1 . The isolated neonatal Wistar rat ventricular cardiomyocytes were cultured and purified with the method of differential attachment .

哮喘豚鼠气道上皮细胞原癌基因c-fos活化与ET-1释放相关性的研究

Study on correlation between c-fos expression and synthesis and release of endothelin-1 ( ET-1 ) in airway epithelial cell in asthmatic guinea-pigs

目的探讨哮喘豚鼠发作时气道上皮细胞原癌基因CFos的活化与上皮细胞释放内皮素1（ET1）的相关性。

Aim To investigate the correlation between c-fos expression and release of endothelin-1 ( ET-1 ) in epithelial cells of airway in asthmatic guinea-pigs .

应用Northern斑点杂交方法研究氯化镉对大鼠肝细胞原癌基因c-fos、c-jun表达的影响。

The effects of cadmium chloride on the expression of proto oncogene c fos and c jun of rat liver cells were studied with Northern dot hybridization .

DFO、5-ALA不同浓度与pH值对大肠癌细胞原卟啉聚集的影响

Effects of DFO , 5-ALA at Different Concentrations and pH Value on the Protoporphyrin Accumulation in Colorectal Cancer Cell

两种细胞原代培养时CFE值差异有非常显著意义（t＝6.78，P＜0.001）。

The difference of the CFE value of the two kinds of primary culture cells was statistically significant ( t = 6.78 , P < 0.001 ) .

目的探讨Wistar大鼠晶体上皮细胞原代培养方法，在形态学方面及晶体蛋白的特异性表达方面，对原代和第二代细胞进行鉴定。

Objective To probe the primary culture method of Wistar rats lens epithelial cells , and identify the morph and crystallins expression of primary and secondary generation cells .

结论联合法更适合湖北钉螺肝脏细胞原代培养，SDH和LDH活性位于肝脏细胞质中。

Conclusion The combination culture method is suitable for primary culture of the cells from O.hupensis liver and the cells show activities of both SDH and LDH in cytoplasm .

P-gp、LRP、MRP、GST-π可作为判断肺癌细胞原发性耐药的指标。

The P-gp , MRP , LRP , GST - π can be an indication to detect the primary drug resistance of lung cancer .

目的：研究人TMJ滑膜细胞原代组织取材和体外培养方法，及其体外生长形态。

Objective : To study the methodology of TMJ synovial membrane cell ( SMC ) culture and the morphological characteristics of SMCs in vitro .

方法用改良MTT法对88例胃癌患者的新鲜标本进行体外肿瘤细胞原代培养和化疗药物敏感性检测，并对其结果进行比较。

Methods The gastric carcinoma cells taken from fresh samples of 88 patients were cultured in vitro to test the sensitivity of these cells to chemotherapeutic drugs using modified MTT method .

[方法]采用体外甲状腺细胞原代培养技术，术中取甲状腺腺瘤旁组织，常规培养7天后，分别加入不同浓度的KI溶液继续培养7天，收集细胞做透射电镜观察。

Follicular cells were cultured for 7 days in different iodine concentrations medium , and then for the transmission electron microscopy ( TEM ) .

已经证实细胞原癌基因c－kit的蛋白产物是造血于细胞因子（SCF）或肥大细胞生长因子（MGF）的细胞膜上受体，对于造血细胞增殖和分化调节起重要作用。

It has been demonstrated that proto-oncogene c-kit protein is the membrane receptor of stem cell factor ( SCF ) or mast cell growth factor ( MGF ) and plays an important role in hemopoietic cell proliferation and differentiation .

采用人胎肝细胞原代培养方法，观察儿茶酚胺和凝血酶对肝细胞富组氨酸糖蛋白（HRG）分泌的影响。

Primary culture of human embryonic hepatocytes was employed to study the effects of catecholamine and thrombin on the secretion of histidine rich glycoprotein ( HRG ) .

结果表明腮腺上皮细胞原代培养4d时成极性排列，形成大小不等的腺腔与导管样结构。

The result showed that the primary culture of parotid gland epithelial cells : cells were in polarity arrangement on the 4th day and formed in different size of acinus and pip kind structure .

将Wistar系大鼠乳鼠心肌细胞原代单层培养5～7d，用组织学方法和生化指标测定方法研究熊胆对缺氧缺糖心肌细胞的影响。

After primary monolayer cultivation of cardiac muscle cells of Wistar suckling mice from 5 to 7 days in vitro , the effects of bear bile for hypoxia and sugar deficiency of cardiac muscle cell were studied by using histochemistry and biochemical methods .

目的建立新生大鼠海马神经细胞原代培养的方法，并利用AnnexinV-PI双染法流式细胞仪检测培养过程中细胞早期凋亡和死亡情况。

Objective : To establish a primary culture method for newborn rat hippocampal cells , and investigate the early stage apoptosis and death of cultured nerve cells using Annexin V-PI staining protocol .

孕妇6项观察指标为：血红蛋白（Hb）、血清铁蛋白（SF）、游离红细胞原卟啉（FEP）、红细胞容积分布宽度（RDW）、平均红细胞容积（MCV）和贫血患病率。

Six observation indices on the pregnant women are : hemoglobin ( Hb ), serum ferritin ( SF ), free erythrocyte protoporphyrin ( FEP ), red cell distribution width ( RDW ), mean corpuscular volum ( MCV ) and anemia prevalence rate .

人膀胱癌相关蛋白（BLCAP）基因是本研究室从细胞原癌、抑癌基因分类芯片中筛选并克隆的宫颈癌候选抑癌基因。

To analyze the cervical carcinoma specimen with an oncogene and anti-oncogene cDNA microarray . We found that Homo sapiens bladder cancer associated protein ( BLCAP ) gene down-expressed .

非受体酪氨酸蛋白激酶Src是最早发现的原癌基因表达蛋白之一。其以两种形式存在：病毒原癌基因表达蛋白v-Src和细胞原癌基因表达蛋白c-Src，两者在进化上具有高度同源性。

The nonreceptor tyrosine kinase c-Src , first identified as an oncogenic protein , has two categories , including virus-Src ( v-Src ) and cell-Src ( c-Src ), Both of which are highly homologous with similar structures and protein tyrosine kinase ( PTK ) activity .

成人角膜缘干细胞原代培养时生长不及胎儿细胞旺盛，6~8d达到生长高峰，CFE为（15.45±3.88）%，随着传代次数增加其增殖能力明显下降；

Adult limbal stem cells grew less swiftly than fetal cells and reached growth peak in 6 ~ 8 days in primary culture when their CFE was ( 15.45 ± 3.88 ) % . Moreover , their proliferating ability declined distinctly along with the passage times increasing .

结果体外培养成人视网膜细胞原代培养可达80d左右，早期培养的细胞中视网膜神经细胞占65%左右，其中可见多数带有外节段的感光细胞。

Results Approximately 65 % of these cells were retinal neural cells , among which photoreceptor cells with lateral segment were observed mainly . After being cultured in vitro for 80 days , the retinal neural cells died away gradually .

胶质细胞原神经营养因子研究进展

Progress in Research of Glial Cell Line - Derived Neurotrophic Factor

晶状体上皮细胞原代培养过程中，细胞生长较为活跃。

Cells were active in the process of LECs primary cultured .

大鼠肾上腺嗜铬细胞原代培养的一种实用方法

A Practical Method for Primary Culture of Rat Adrenal Chromaffin Cells

铁离子螯合剂去铁敏在大鼠心肌细胞原代培养中对心肌细胞的保护作用

Protective Effect of Deferoxamine on Primary Culture of Cardiomyocytes in Rats